Keratin 6 antibody

Catalog No. ABIN599805

Product Details anti-Keratin 6 Antibody

Target: Keratin 6 (KRT6)

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This Keratin 6 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: Recognizes human Keratin 6. Species cross-reactivity: Mouse.

Purification: Protein A purified

Immunogen: Keratin 6 of human callus cytoskeletal preparation.

Clone: KS6-KA12

Isotype: IgG1

Application Details

Application Notes: Approved: IHC, IHC-Fr (1:10), IHC-P (1:10), WB (1:10)

Usage: Suitable for discrimination of keratinizing and non-keratinizing squamous cell carcinoma versus e. g. poorly differentiated adenocarcinoma. Applications: Suitable for use in Western Blot and Immunohistochemistry. Western Blot: Reacts with Mr 56000 polypeptide of Keratin 6 of human squamous epithelia. Immunohistochemistry (Frozen, paraffin): 1:10 diluted with PBS using the ABC method. Incubate for 1 hour at RT, extended for paraffin sections. Fixation with formalin is recommended. Microwave treatment improves performance.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris,  pH 7.4, 0.5 % BSA, 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Aliquot to Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: May be stored at 4°C for short-term only. Aliquot to avoid freeze-thaw cycles. Store at -20°C. Aliquots are stable for at least 1 year.

Target Details for Keratin 6

Target: Keratin 6 (KRT6)

Alternative Name: KRT6 / CK6 / Cytokeratin 6 (KRT6 Products)

Synonyms: Krt2-6 antibody, Krt-2.6 antibody, keratin 6 antibody, Krt6 antibody