MHC Class II (RT1Bu) antibody (PE)
Reactivity: Rat, Mouse
FACS
Host: Mouse
Monoclonal
OX-3
PE
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- Target
- MHC Class II (RT1Bu)
- Reactivity
- Rat, Mouse
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- PE
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Application
- Flow Cytometry (FACS)
- Specificity
- Recognizes a polymorphic determinant of rat Ia antigen (RT1. Bu) present on Lewis, Wistar and AO strain rats but not BN, DA or PVG/c strains. Is useful for distinguishing Ia positive cells from different rat strains, e. g. for recognizing cells of donor origin in bone marrow reconstituted radiation chimaeras. MRC OX-3 Reacts with mouse strains of MHC haplotypes b and s, and analysis of recombinant mouse strains showed that the determinants mapped to the I-A region. Recognizes Ia antigens on B-cells, dendritic cells and certain epithelial cells. This product is routinely tested in flow cytometry on Lewis rat splenocytes.
- Purification
- Ion exchange chromatography
- Immunogen
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Rat thymocyte membrane glycoproteins.
Type of Immunogen: Membrane extract - Clone
- OX-3
- Isotype
- IgG1
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- Application Notes
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Approved: Flo (1:1)
Usage: Flow Cytometry: Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Method sheets are available on request. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. - Comment
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Target Species of Antibody: Rat
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Distilled Water.
- Concentration
- Lot specific
- Buffer
- Lyophilized, PBS, pH 7.4, 0.09 % sodium azide, 1 % BSA.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C
- Storage Comment
- 4°C, avoid freezing.
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- Target
- MHC Class II (RT1Bu)
- Alternative Name
- MHC Class II RT-1Bu
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