Clathrin Heavy Chain (CLTC) (AA 1617-1630) antibody

Catalog No. ABIN574100

Product Details

Target: Clathrin Heavy Chain (CLTC)

Binding Specificity: AA 1617-1630

Reactivity: Human, Mouse, Rat, Cow, Pig, Dog, Monkey, Xenopus laevis, Chicken, Horse, Rabbit, Zebrafish (Danio rerio), Bat

Host: Goat

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human CLTC.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bat, Bovine, Horse, Rabbit, Pig, Opossum, Turkey, Chicken, Platypus, Xenopus, Zebrafish (100%) Stickleback (93%) Pufferfish (86%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-ESLRKEEEQATETQ from an internal region (near C-terminus) of human CLTC (NP_004850.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bat, Bovine, Horse, Rabbit, Pig, Opossum, Turkey, Chicken, Platypus, Xenopus, Zebrafish (100%), Stickleback (93%), Pufferfish (86%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (3.75 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 3.75 μg/mL

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details

Target: Clathrin Heavy Chain (CLTC)

Alternative Name: CLTC / Clathrin (CLTC Products)

Background: Name/Gene ID: CLTC

Synonyms: CLTC, Clathrin, heavy chain, CLH17, CLTCL2, CHC, CHC17, Clathrin heavy chain 1, CLH-17, KIAA0034, Hc, Clathrin Heavy Chain, Clathrin, heavy chain (Hc)

Gene ID: 1213

NCBI Accession: NP_004850

UniProt: Q00610