DLGAP1 antibody (AA 391-405)

Catalog No. ABIN574097

Product Details anti-DLGAP1 Antibody

Target: DLGAP1 (Discs, Large (Drosophila) Homolog-Associated Protein 1 (DLGAP1))

Binding Specificity: AA 391-405

Reactivity: Human, Mouse, Rat, Dog, Hamster, Horse, Rabbit, Xenopus laevis, Bat, Cow

Host: Goat

Clonality: Polyclonal

Conjugate: This DLGAP1 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human DLGAP1. This antibody is expected to recognize both reported isoforms (NP_001003809.1, NP_004737.2)

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Dog, Bat, Horse, Rabbit, Opossum, Xenopus (100%) Turkey, Chicken, Seabass, Pufferfish, Zebrafish (93%) Stickleback (87%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-KISNEHSPKLQIRSH from an internal region of human DLGAP1 (NP_004737.2, NP_001003809.1). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Dog, Bat, Horse, Rabbit, Opossum, Xenopus (100%), Turkey, Chicken, Seabass, Pufferfish, Zebrafish (93%), Stickleback (87%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (3.75 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 3.75 μg/mL

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for DLGAP1

Target: DLGAP1 (Discs, Large (Drosophila) Homolog-Associated Protein 1 (DLGAP1))

Alternative Name: DLGAP1 / SAPAP1 / GKAP (DLGAP1 Products)

Synonyms: DKFZp469D0713 antibody, gkap antibody, sapap1 antibody, DAP-1 antibody, DAP-1-ALPHA antibody, DAP-1-BETA antibody, GKAP antibody, SAPAP1 antibody, hGKAP antibody, 4933422O14Rik antibody, 9630002F18 antibody, AI845682 antibody, AI848168 antibody, BB075781 antibody, D17Bwg0511e antibody, GKAP/SAPAP antibody, GKPA/SAPAP antibody, Gkap antibody, Sapap1 antibody, mKIAA4162 antibody, DLG associated protein 1 antibody, discs, large (Drosophila) homolog-associated protein 1 antibody, disks large-associated protein 1 antibody, discs, large homolog-associated protein 1 antibody, discs, large (Drosophila) homolog-associated protein 1b antibody, DLGAP1 antibody, LOC100408093 antibody, LOC100548434 antibody, dlgap1 antibody, Dlgap1 antibody, dlgap1b antibody

Background: Name/Gene ID: DLGAP1

Synonyms: DLGAP1, DAP-1, DAP-1-ALPHA, HGKAP, PSD-95/SAP90 binding protein 1, PSD-95/SAP90-binding protein 1, SAPAP1, DAP-1-BETA, DAP1, GKAP

Gene ID: 9229

NCBI Accession: NP_004737, NP_001003809

UniProt: O14490