Septin 4 antibody (AA 425-435)

Catalog No. ABIN571248

Product Details anti-Septin 4 Antibody

Target: Septin 4 (SEPT4)

Binding Specificity: AA 425-435

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Septin 4 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Purpose: SEPT4 (aa425-435)

Sequence: RNKLTRESGT D

Specificity: This antibody is expected to recognize isoform 1 (NP_004565.1), 3 (NP_536341.1) and 4 (NP_001185642.1). Amino acid numbering in name refers to NP_004565.1 sequence.

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: C-RNKLTRESGTD

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Brain (Cerebellum). Recommended concentration: 3.75 μg/mL.

Western Blot: Approx 50 kDa band observed in Human Heart lysates and in preliminary testing of Mouse Brain lysate (calculated MW of 53.0 kDa according to NP_536341.1). Recommended concentration: 1-3 μg/mL. Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:64000.

Comment:

Flow Cytometry: Flow cytometric analysis of Jurkat cells. Recommended concentration: 10ug/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for Septin 4

Target: Septin 4 (SEPT4)

Alternative Name: SEPT4 (SEPT4 Products)

Synonyms: EG3-1RVC antibody, EG3RVC antibody, Pnutl2 antibody, SEPT4 antibody, PNUTL2 antibody, DKFZp459I029 antibody, DKFZp459F1329 antibody, ARTS antibody, BRADEION antibody, CE5B3 antibody, H5 antibody, MART antibody, SEP4 antibody, hCDCREL-2 antibody, Bh5 antibody, Gm11492 antibody, OTTMUSG00000001265 antibody, RP23-168C20.1 antibody, septin 4 antibody, uncharacterized protein C17orf47 homolog antibody, Sept4 antibody, SEPT4 antibody, PAAG_00317 antibody, PGTG_11810 antibody, LOC100012173 antibody

Background: SEPT4, septin 4, ARTS, BRADEION, CE5B3, H5, MART, PNUTL2, SEP4, hCDCREL-2, hucep-7, cell division control-related protein 2, cerebral protein 7, peanut-like 2, septin-M

Gene ID: 5414, 18952

NCBI Accession: NP_004565, NP_536341, NP_001185642