PLA2G4A antibody (Internal Region)

Catalog No. ABIN571180

Product Details anti-PLA2G4A Antibody

Target: PLA2G4A (Phospholipase A2, Group IVA (Cytosolic, Calcium-Dependent) (PLA2G4A))

Binding Specificity: Internal Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This PLA2G4A antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Purpose: PLA2G4A

Sequence: QEKTFRQQRK EHIR

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-QEKTFRQQRKEHIR, from the internal region of the protein sequence according to NP_077734.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Uterus. Recommended concentration: 3.75 μg/mL.

Western Blot: Approx 80-85 kDa band observed in lysates of cell line A549 (calculated MW of 85.2 kDa according to NP_077734.1). Preliminary experiments also showed an 85- 90 kDa band in lysates of cell lines A431, HeLa and NIH-3T3 Recommended concentration:

Peptide ELISA: antibody detection limit dilution 1:32000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of A549 cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of A549 cells. Recommended concentration: 10ug/ml.<

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for PLA2G4A

Target: PLA2G4A (Phospholipase A2, Group IVA (Cytosolic, Calcium-Dependent) (PLA2G4A))

Alternative Name: PLA2G4A (PLA2G4A Products)

Synonyms: PLA2G4 antibody, cPLA2-alpha antibody, Pla2c antibody, Pla2g4 antibody, cPLA2 antibody, pla2g4 antibody, CPLA2 antibody, PLA2G4A antibody, PLA2 antibody, PLA2G1B antibody, cPLA2alpha antibody, pla2g4a antibody, si:dkey-97o5.1 antibody, phospholipase A2 group IVA antibody, phospholipase A2 group IVA L homeolog antibody, phospholipase A2, group IVA (cytosolic, calcium-dependent) antibody, phospholipase A2, group IVAb (cytosolic, calcium-dependent) antibody, PLA2G4A antibody, Pla2g4a antibody, pla2g4a.L antibody, pla2g4ab antibody

Background: PLA2G4A, phospholipase A2, group IVA (cytosolic, calcium-dependent), MGC126350, PLA2G4, cPLA2-alpha, calcium-dependent phospholipid-binding protein, cytosolic phospholipase A2, group IVA, lysophospholipase, phosphatidylcholine 2-acylhydrolase

Gene ID: 5321

NCBI Accession: NP_077734

Pathways: Inositol Metabolic Process, G-protein mediated Events, VEGF Signaling