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Dystroglycan antibody (Internal Region)

Verified DAG1 Reactivity: Human WB, ELISA, IHC, IF, FACS Host: Goat Polyclonal unconjugated
Catalog No. ABIN571179
  • Target See all Dystroglycan (DAG1) Antibodies
    Dystroglycan (DAG1) (Dystroglycan 1 (DAG1))
    Binding Specificity
    • 22
    • 12
    • 10
    • 4
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Internal Region
    Reactivity
    • 37
    • 22
    • 20
    • 5
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Human
    Host
    • 51
    • 4
    • 2
    Goat
    Clonality
    • 53
    • 4
    Polyclonal
    Conjugate
    • 22
    • 6
    • 4
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This Dystroglycan antibody is un-conjugated
    Application
    • 44
    • 22
    • 20
    • 13
    • 13
    • 8
    • 5
    • 4
    • 4
    • 3
    • 2
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)
    Purpose
    DAG1
    Sequence
    HVGKHEYFMH ATDK
    Specificity
    This antibody is expected to recognize both the precursor and the mature alpha-dystroglycan, but not the mature beta-dystroglycan.
    Cross-Reactivity
    Human
    Purification
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    Grade
    Verified
    Immunogen
    C-HVGKHEYFMHATDK
    Isotype
    IgG
    Top Product
    Discover our top product DAG1 Primary Antibody
  • Application Notes

    Immunohistochemistry: Paraffin embedded Human Heart. Recommended concentration: 5 μg/mL.

    Western Blot: Approx 100 kDa band observed in Human Skeletal Muscle lysates (calculated MW of 97.4 kDa according to Human NP_004384.4 ). Recommended concentration: 0.1-0.3 μg/mL. Primary incubation 1 hour at room temperature.

    Peptide ELISA: antibody detection limit dilution 1:128000.

    Comment

    Immunofluorescence: Strong expression of the protein seen in the ER and cytoplasm of HeLa and U2OS cells. Recommended concentration: 10μg/ml.

    Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentr

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.5 mg/mL
    Buffer
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Minimize freezing and thawing.
    Storage
    -20 °C
    Storage Comment
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Target
    Dystroglycan (DAG1) (Dystroglycan 1 (DAG1))
    Alternative Name
    DAG1 (DAG1 Products)
    Synonyms
    LOC398500 antibody, dag1 antibody, MGC53537 antibody, 156DAG antibody, A3a antibody, AGRNR antibody, DAG antibody, MDDGC7 antibody, MDDGC9 antibody, DAG1 antibody, RAB7 antibody, dg antibody, a3a antibody, dag antibody, agrnr antibody, 156dag antibody, D9Wsu13e antibody, DG antibody, Dp427 antibody, Dp71 antibody, CG18250 antibody, CT41273 antibody, DmDG antibody, Dmel\\CG18250 antibody, atu antibody, dgn antibody, dys antibody, GB14967 antibody, APOJ antibody, CLI antibody, RATTRPM2B antibody, SGP-2 antibody, SGP2 antibody, SP-40 antibody, SP40 antibody, TRPM-2 antibody, TRPM2B antibody, Trpm2 antibody, Trpmb antibody, Ala-1 antibody, H9/25 antibody, Ly-27 antibody, Ly-6 antibody, Ly27 antibody, wu:fb83d06 antibody, wu:fi25f06 antibody, wu:fi37b08 antibody, zgc:109786 antibody, DystroGlycaN antibody, dystroglycan 1 L homeolog antibody, dystroglycan 1 antibody, dystroglycan 1 S homeolog antibody, Dystroglycan antibody, dystroglycan antibody, clusterin antibody, lymphocyte antigen 6 complex antibody, dgn-1 antibody, dag1.L antibody, dgn-2 antibody, dgn-3 antibody, DAG1 antibody, dag1.S antibody, Dag1 antibody, dag1 antibody, Dg antibody, LOC408826 antibody, Clu antibody, Ly6 antibody
    Background
    DAG1, dystroglycan 1 (dystrophin-associated glycoprotein 1), 156DAG, A3a, AGRNR, DAG, alpha-dystroglycan, beta-dystroglycan, dystroglycan 1
    Gene ID
    1605
    NCBI Accession
    NP_004384
    Pathways
    Maintenance of Protein Location, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus
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