Dystroglycan antibody (Internal Region)

Catalog No. ABIN571179

Product Details anti-Dystroglycan Antibody

Target: Dystroglycan (DAG1) (Dystroglycan 1 (DAG1))

Binding Specificity: Internal Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Dystroglycan antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Purpose: DAG1

Sequence: HVGKHEYFMH ATDK

Specificity: This antibody is expected to recognize both the precursor and the mature alpha-dystroglycan, but not the mature beta-dystroglycan.

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: C-HVGKHEYFMHATDK

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Heart. Recommended concentration: 5 μg/mL.

Western Blot: Approx 100 kDa band observed in Human Skeletal Muscle lysates (calculated MW of 97.4 kDa according to Human NP_004384.4 ). Recommended concentration: 0.1-0.3 μg/mL. Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:128000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the ER and cytoplasm of HeLa and U2OS cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentr

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for Dystroglycan

Target: Dystroglycan (DAG1) (Dystroglycan 1 (DAG1))

Alternative Name: DAG1 (DAG1 Products)

Synonyms: LOC398500 antibody, dag1 antibody, MGC53537 antibody, 156DAG antibody, A3a antibody, AGRNR antibody, DAG antibody, MDDGC7 antibody, MDDGC9 antibody, DAG1 antibody, RAB7 antibody, dg antibody, a3a antibody, dag antibody, agrnr antibody, 156dag antibody, D9Wsu13e antibody, DG antibody, Dp427 antibody, Dp71 antibody, CG18250 antibody, CT41273 antibody, DmDG antibody, Dmel\\CG18250 antibody, atu antibody, dgn antibody, dys antibody, GB14967 antibody, APOJ antibody, CLI antibody, RATTRPM2B antibody, SGP-2 antibody, SGP2 antibody, SP-40 antibody, SP40 antibody, TRPM-2 antibody, TRPM2B antibody, Trpm2 antibody, Trpmb antibody, Ala-1 antibody, H9/25 antibody, Ly-27 antibody, Ly-6 antibody, Ly27 antibody, wu:fb83d06 antibody, wu:fi25f06 antibody, wu:fi37b08 antibody, zgc:109786 antibody, DystroGlycaN antibody, dystroglycan 1 L homeolog antibody, dystroglycan 1 antibody, dystroglycan 1 S homeolog antibody, Dystroglycan antibody, dystroglycan antibody, clusterin antibody, lymphocyte antigen 6 complex antibody, dgn-1 antibody, dag1.L antibody, dgn-2 antibody, dgn-3 antibody, DAG1 antibody, dag1.S antibody, Dag1 antibody, dag1 antibody, Dg antibody, LOC408826 antibody, Clu antibody, Ly6 antibody

Background: DAG1, dystroglycan 1 (dystrophin-associated glycoprotein 1), 156DAG, A3a, AGRNR, DAG, alpha-dystroglycan, beta-dystroglycan, dystroglycan 1

Gene ID: 1605

NCBI Accession: NP_004384

Pathways: Maintenance of Protein Location, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus