POU3F2 antibody (Internal Region)

Catalog No. ABIN571160

Product Details anti-POU3F2 Antibody

Target: POU3F2 (POU Class 3 Homeobox 2 (POU3F2))

Binding Specificity: Internal Region

Reactivity: Human, Rat, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This POU3F2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purpose: POU3F2 / BRN2 / OCT7

Sequence: AQSLVQGDYG ALQSN

Cross-Reactivity: Cow, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-AQSLVQGDYGALQSN, from the internal region (near N Terminus) of the protein sequence according to NP_005595.2.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 45 kDa band observed in Human Brain (Cerebellum) and in Mouse and Rat Brain lysates (calculated MW of 46.9 kDa according to Human NP_005595.2 and 47.1 kDa according to Mouse NP_032925.1). Recommended concentration: 0.1-0.3 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:8000.

Comment:

Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for POU3F2

Target: POU3F2 (POU Class 3 Homeobox 2 (POU3F2))

Alternative Name: POU3F2 (POU3F2 Products)

Synonyms: BRN2 antibody, N-Oct3 antibody, OCT7 antibody, OTF-7 antibody, OTF7 antibody, POUF3 antibody, brn-2 antibody, oct-7 antibody, XlPOU3 antibody, pou3f2 antibody, pou3f2-A antibody, pou3f4 antibody, 9430075J19Rik antibody, A230098E07Rik antibody, Brn-2 antibody, Brn2 antibody, Otf7 antibody, XLPOU3b antibody, pou47 antibody, zp47 antibody, zp47pou antibody, brn-1.2 antibody, brn1.2 antibody, id:ibd5088 antibody, POU class 3 homeobox 2 antibody, POU class 3 homeobox 2 L homeolog antibody, POU domain, class 3, transcription factor 2 antibody, POU class 3 homeobox 2 S homeolog antibody, POU class 3 homeobox 2b antibody, POU class 3 homeobox 2a antibody, POU3F2 antibody, pou3f2.L antibody, Pou3f2 antibody, pou3f2.S antibody, pou3f2b antibody, pou3f2a antibody

Background: POU3F2, POU class 3 homeobox 2, BRN2, OCT7, OTF7, POUF3, POU domain, class 3, transcription factor 2

Gene ID: 5454, 18992, 29588

NCBI Accession: NP_005595