SPON2 antibody (Internal Region)

Catalog No. ABIN571150

Product Details anti-SPON2 Antibody

Target: SPON2 (Spondin 2 (SPON2))

Binding Specificity: Internal Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This SPON2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purpose: Spondin 2 / Mindin

Sequence: RKNQYVSNGL RDFA

Specificity: Reported variants NP_036577.1 and NP_001121797.1 represent identical protein.

Cross-Reactivity: Cow, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Recent

Immunogen: Peptide with sequence C-RKNQYVSNGLRDFA, from the internal region of the protein sequence according to NP_036577.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 35 kDa band observed in preliminary testing of Human and Mouse Prostate lysate after 1 μg/mL antibody staining (calculated MW of 35.8 kDa according to Human NP_036577.1). Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for SPON2

Target: SPON2 (Spondin 2 (SPON2))

Alternative Name: SPON2 (SPON2 Products)

Background: SPON2, spondin 2, extracellular matrix protein, DIL-1, DIL1, M-spondin, Mindin

Gene ID: 10417

NCBI Accession: NP_036577