ACO2 antibody (Internal Region)

Catalog No. ABIN571124

Product Details anti-ACO2 Antibody

Target: ACO2 (Aconitase 2, Mitochondrial (ACO2))

Binding Specificity: Internal Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This ACO2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Purpose: Aconitase 2

Sequence: QHVDVSPTSQ RLQ

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-QHVDVSPTSQRLQ, from the internal region of the protein sequence according to NP_001089.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Breast shows mitochondrial staining in the epithelial cells of the ducts. Recommended concentration, 3-5 μg/mL.

Western Blot: Approx 90 kDa band observed in Human Heart lysates (calculated MW of 85.4 kDa according to NP_001089.1). Recommended concentration: 0.01-0.03 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:32000.

Comment:

Immunofluorescence: Customer finds weak pixulate staining in the cytoplasm of HeLa cells.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for ACO2

Target: ACO2 (Aconitase 2, Mitochondrial (ACO2))

Alternative Name: ACO2 (ACO2 Products)

Synonyms: DDBDRAFT_0206187 antibody, DDBDRAFT_0230168 antibody, DDB_0206187 antibody, DDB_0230168 antibody, aconitase 2 antibody, F10M23.310 antibody, F10M23_310 antibody, ACONM antibody, ICRD antibody, Aco-2 antibody, Aco3 antibody, D10Wsu183e antibody, cb1017 antibody, wu:fa10e03 antibody, wu:fb69g04 antibody, wu:fc20c11 antibody, aconitase 2 antibody, aconitase 2 S homeolog antibody, aconitate hydratase, mitochondrial antibody, aconitase, mitochondrial antibody, mitochondrial aconitate hydratase antibody, aconitase 2, mitochondrial antibody, Probable aconitate hydratase, mitochondrial antibody, ACO2 antibody, aco2.S antibody, Bm1_07420 antibody, aco2 antibody, ach1 antibody, Aco2 antibody, aco-2 antibody

Background: ACO2, aconitase 2, mitochondrial, ACONM, MGC20605, MGC33908, aconitate hydratase, citrate hydro-lyase

Gene ID: 50, 11429, 79250

NCBI Accession: NP_001089