CHCHD3 antibody (AA 151-164)

Catalog No. ABIN571117

Product Details anti-CHCHD3 Antibody

Target: CHCHD3 (Coiled-Coil-Helix-Coiled-Coil-Helix Domain Containing 3 (CHCHD3))

Binding Specificity: AA 151-164

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This CHCHD3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Purpose: CHCHD3 (aa151-164)

Sequence: RSSEFYRVTT EQYQ

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat, Sheep

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-RSSEFYRVTTEQYQ, from the internal region of the protein sequence according to NP_060282.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Kidney. Recommended concentration: 5 μg/mL.

Western Blot: Approx 26.2 kDa band observed in Human Placenta lysates (calculated MW of 26 kDa according to NP_060282.1). Recommended concentration: 0.3-1 μg/mL. A customer reported ~30 kDa in THP1 and in human primary monocyte derived dendritic cells.

Peptide ELISA: antibody detection limit dilution 1:32000.

Comment:

Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for CHCHD3

Target: CHCHD3 (Coiled-Coil-Helix-Coiled-Coil-Helix Domain Containing 3 (CHCHD3))

Alternative Name: CHCHD3 (CHCHD3 Products)

Synonyms: MINOS3 antibody, PPP1R22 antibody, 0610041L09Rik antibody, 1700039J09Rik antibody, AW558177 antibody, plxna4 antibody, FLJ20420 antibody, wu:fe23h05 antibody, zgc:111837 antibody, si:rp71-18a24.1 antibody, MGC80265 antibody, MGC88973 antibody, coiled-coil-helix-coiled-coil-helix domain containing 3 antibody, coiled-coil-helix-coiled-coil-helix domain containing 3a antibody, coiled-coil-helix-coiled-coil-helix domain containing 3 L homeolog antibody, CHCHD3 antibody, Chchd3 antibody, chchd3a antibody, chchd3.L antibody, chchd3 antibody, LOC100229580 antibody

Background: CHCHD3, coiled-coil-helix-coiled-coil-helix domain containing 3, FLJ20420

Gene ID: 54927, 66075, 296966

NCBI Accession: NP_060282