Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

Tppp antibody (Internal Region)

Verified Tppp Reactivity: Human, Mouse, Rat WB, ELISA, IHC Host: Goat Polyclonal unconjugated
Catalog No. ABIN571099
  • Target See all Tppp Antibodies
    Tppp (Tubulin Polymerization Promoting Protein (Tppp))
    Binding Specificity
    • 15
    • 7
    • 7
    • 6
    • 6
    • 6
    • 4
    • 4
    • 4
    • 2
    • 1
    Internal Region
    Reactivity
    • 40
    • 34
    • 16
    • 7
    • 6
    • 4
    • 4
    • 4
    • 3
    • 2
    • 1
    Human, Mouse, Rat
    Host
    • 54
    • 3
    Goat
    Clonality
    • 55
    • 2
    Polyclonal
    Conjugate
    • 26
    • 4
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This Tppp antibody is un-conjugated
    Application
    • 50
    • 24
    • 13
    • 13
    • 8
    • 5
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
    Purpose
    TPPP/p25
    Sequence
    DPSKDRAAKR LSLES
    Cross-Reactivity
    Human
    Purification
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    Grade
    Verified
    Immunogen
    C-DPSKDRAAKRLSLES
    Isotype
    IgG
  • Application Notes

    Immunohistochemistry: In paraffin embedded Human Cerebellum shows strong staining of axonal bundles through the granular layer. Recommended concentration: 1-2 μg/mL. Paraffin embedded Human Cortex. Recommended concentration: 3.75 μg/mL.

    Western Blot: Approx 26 kDa band observed in Human Brain (Cerebellum), Mouse and Rat Brain lysates (calculated MW of 23.7 kDa according to NP_008961.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (

    Peptide ELISA: antibody detection limit dilution 1:32000.

    Comment

    Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.5 mg/mL
    Buffer
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Minimize freezing and thawing.
    Storage
    -20 °C
    Storage Comment
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Target
    Tppp (Tubulin Polymerization Promoting Protein (Tppp))
    Alternative Name
    TPPP (Tppp Products)
    Synonyms
    TPPP/p25 antibody, TPPP1 antibody, p24 antibody, p25 antibody, p25alpha antibody, 2900041A09Rik antibody, AI849835 antibody, RGD1310121 antibody, tubulin polymerization promoting protein antibody, TPPP antibody, Tppp antibody
    Background
    TPPP, tubulin polymerization promoting protein, TPPP/p25, TPPP1, p24, p25, p25alpha, brain specific protein p25 alpha, glycogen synthase kinase 3 (GSK3) inhibitor p24, tubulin polymerization-promoting protein
    Gene ID
    11076, 72948, 361466
    NCBI Accession
    NP_008961
You are here:
Support