Tppp antibody (Internal Region)
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- Target See all Tppp Antibodies
- Tppp (Tubulin Polymerization Promoting Protein (Tppp))
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Binding Specificity
- Internal Region
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Reactivity
- Human, Mouse, Rat
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- This Tppp antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
- Purpose
- TPPP/p25
- Sequence
- DPSKDRAAKR LSLES
- Cross-Reactivity
- Human
- Purification
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Grade
- Verified
- Immunogen
- C-DPSKDRAAKRLSLES
- Isotype
- IgG
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- Application Notes
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Immunohistochemistry: In paraffin embedded Human Cerebellum shows strong staining of axonal bundles through the granular layer. Recommended concentration: 1-2 μg/mL. Paraffin embedded Human Cortex. Recommended concentration: 3.75 μg/mL.
Western Blot: Approx 26 kDa band observed in Human Brain (Cerebellum), Mouse and Rat Brain lysates (calculated MW of 23.7 kDa according to NP_008961.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (
Peptide ELISA: antibody detection limit dilution 1:32000.
- Comment
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Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Minimize freezing and thawing.
- Storage
- -20 °C
- Storage Comment
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- Target
- Tppp (Tubulin Polymerization Promoting Protein (Tppp))
- Alternative Name
- TPPP (Tppp Products)
- Synonyms
- TPPP/p25 antibody, TPPP1 antibody, p24 antibody, p25 antibody, p25alpha antibody, 2900041A09Rik antibody, AI849835 antibody, RGD1310121 antibody, tubulin polymerization promoting protein antibody, TPPP antibody, Tppp antibody
- Background
- TPPP, tubulin polymerization promoting protein, TPPP/p25, TPPP1, p24, p25, p25alpha, brain specific protein p25 alpha, glycogen synthase kinase 3 (GSK3) inhibitor p24, tubulin polymerization-promoting protein
- Gene ID
- 11076, 72948, 361466
- NCBI Accession
- NP_008961
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