SRGAP2 antibody (Internal Region)

Catalog No. ABIN571090

Product Details anti-SRGAP2 Antibody

Target: SRGAP2 (SLIT-ROBO rho GTPase Activating Protein 2 (SRGAP2))

Binding Specificity: Internal Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This SRGAP2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Purpose: SRGAP2

Sequence: KQEDRQTPRS PDST

Specificity: This antibody is expected to recognize isoform a (NP_056141.2).

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-KQEDRQTPRSPDST, from the internal region of the protein sequence according to NP_056141.2.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 124 kDa band observed in lysates of cell line HeLa and in rat primary culture cortical neurons at Day 4 (calculated MW of 121 kDa according to human NP_056141.2 but the rat protein sequence is stlll provisional). Recommended concentrat

Peptide ELISA: antibody detection limit dilution 1:8000.

Comment:

Immunofluorescence: Transfected HEK293 transiently expressing GFP-tagged Human srGAP2 with the primary antibody stained in the red channel (Confocal IF).

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for SRGAP2

Target: SRGAP2 (SLIT-ROBO rho GTPase Activating Protein 2 (SRGAP2))

Alternative Name: SRGAP2 (SRGAP2 Products)

Background: SRGAP2, SLIT-ROBO Rho GTPase activating protein 2, FNBP2, KIAA0456, srGAP3, formin binding protein 2

Gene ID: 23380, 14270, 360840

NCBI Accession: NP_056141