Deltex Homolog 3 antibody (Internal Region)

Catalog No. ABIN571068

Product Details anti-Deltex Homolog 3 Antibody

Target: Deltex Homolog 3 (DTX3) (Deltex Homolog 3 (Drosophila) (DTX3))

Binding Specificity: Internal Region

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This Deltex Homolog 3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Purpose: DTX3

Sequence: KNKVTVSKPV WD

Cross-Reactivity: Cow, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-KNKVTVSKPVWD, from the internal region (near N Terminus) of the protein sequence according to NP_848597.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Brain (Cortex). Recommended concentration: 10 μg/mL.

Western Blot: Approx 90 kDa band observed in Mouse Fetal Brain lysates and in lysates of cell line NIH3T3 (calculated MW of 38.0 kDa according to Human NP_848597.1 and 37.98 kDa to Mouse NP_109639.1). The observed much higher molecular weight corresponds to

Peptide ELISA: antibody detection limit dilution 1:8000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for Deltex Homolog 3

Target: Deltex Homolog 3 (DTX3) (Deltex Homolog 3 (Drosophila) (DTX3))

Alternative Name: DTX3 (DTX3 Products)

Synonyms: RGD1566181 antibody, Deltex3 antibody, mg310 antibody, RNF154 antibody, deltex E3 ubiquitin ligase 3 antibody, deltex homolog 3 (Drosophila) antibody, deltex 3, E3 ubiquitin ligase antibody, Dtx3 antibody, DTX3 antibody, dtx3 antibody

Background: DTX3, deltex 3 homolog (Drosophila), FLJ34766, MGC138863, MGC138864, RNF154, deltex 3 homolog

Gene ID: 196403, 80904, 500847

NCBI Accession: NP_848597

Pathways: Notch Signaling