FAS antibody (Internal Region)
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- Target See all FAS Antibodies
- FAS (TNF Receptor Superfamily, Member 6 (FAS))
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Binding Specificity
- Internal Region
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Reactivity
- Human
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- This FAS antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), ELISA
- Purpose
- FAS / CD95
- Sequence
- KTCRKHRKEN QGSH
- Specificity
- This antibody is expected to recognize reported isoforms 1 (NP_000034.1), 2 (NP_690610.1) and 3 (NP_690611.1).
- Cross-Reactivity
- Human
- Purification
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Grade
- Verified
- Immunogen
- Peptide with sequence KTCRKHRKENQGSH, from the internal region of the protein sequence according to NP_000034.1, NP_690610.1, NP_690611.1.
- Isotype
- IgG
- Top Product
- Discover our top product FAS Primary Antibody
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- Application Notes
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Immunohistochemistry: Paraffin embedded Human Tonsil and Small Intestine. Recommended concentration: 3.75 μg/mL.
Western Blot: Approx 37 kDa band observed in lysates of cell line MOLT4 (calculated MW of 37.7 kDa according to NP_000034.1). Recommended concentration: 0.1-0.3 μg/mL. Primary incubation was 1 hour.
Peptide ELISA: antibody detection limit dilution 1:32000.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Minimize freezing and thawing.
- Storage
- -20 °C
- Storage Comment
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- Target
- FAS (TNF Receptor Superfamily, Member 6 (FAS))
- Alternative Name
- FAS (FAS Products)
- Synonyms
- ALPS1A antibody, APO-1 antibody, APT1 antibody, CD95 antibody, FAS1 antibody, FASTM antibody, TNFRSF6 antibody, AI196731 antibody, APO1 antibody, TNFR6 antibody, Tnfrsf6 antibody, lpr antibody, Fas cell surface death receptor antibody, Fas (TNF receptor superfamily member 6) antibody, FAS antibody, Fas antibody, fas antibody
- Background
- FAS, Fas (TNF receptor superfamily, member 6), ALPS1A, APO-1, APT1, CD95, FAS1, FASTM, TNFRSF6, APO-1 cell surface antigen, CD95 antigen, Fas AMA, Fas antigen, OTTHUMP00000020045, OTTHUMP00000020046, OTTHUMP00000020051, OTTHUMP00000059646, apoptosis antig
- Gene ID
- 355
- NCBI Accession
- NP_000034, NP_690610, NP_690611
- Pathways
- p53 Signaling, Apoptosis, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity
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