EIF5A antibody (Internal Region)

Catalog No. ABIN571047

Product Details anti-EIF5A Antibody

Target: EIF5A (Eukaryotic Translation Initiation Factor 5A (EIF5A))

Binding Specificity: Internal Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This EIF5A antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Purpose: eIF5A

Sequence: RPPHRASFLK RLESK

Specificity: Reported variants NP_001137233.1, NP_001961.1 and NP_001137234.1 represent identical protein:

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-RPPHRASFLKRLESK, from the internal region (near N Terminus) of the protein sequence according to NP_001137232.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Spleen, Tonsil and Vessel. Recommended concentration: 3.75 μg/mL.

Western Blot: Approx 20 kDa band observed in MOLT4 lysates (calculated MW of 20.2 kDa according to NP_001137232.1). Recommended concentration: 1-3 μg/mL. Primary incubation was 1 hour. This antibody has been successfully used in WB on Human, PMID: 27414022

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for EIF5A

Target: EIF5A (Eukaryotic Translation Initiation Factor 5A (EIF5A))

Alternative Name: EIF5A (EIF5A Products)

Background: EIF5A, eukaryotic translation initiation factor 5A, EIF-5A, EIF5A1, MGC104255, MGC99547, eIF5AI, OTTHUMP00000128384, uORF A

Gene ID: 1984

NCBI Accession: NP_001137232

Pathways: Regulation of Muscle Cell Differentiation