TNFSF14 antibody (Internal Region)
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- Target See all TNFSF14 Antibodies
- TNFSF14 (Tumor Necrosis Factor (Ligand) Superfamily, Member 14 (TNFSF14))
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Binding Specificity
- Internal Region
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Reactivity
- Human
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- This TNFSF14 antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Immunofluorescence (IF)
- Purpose
- LIGHT / CD258
- Sequence
- HEVNPAAHLT GANS
- Specificity
- This antibody is expected to recognize both reported isoforms (NP_003798.2, NP_742011.1).
- Cross-Reactivity
- Human
- Purification
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Grade
- Verified
- Immunogen
- C-HEVNPAAHLTGANS
- Isotype
- IgG
- Top Product
- Discover our top product TNFSF14 Primary Antibody
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- Application Notes
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Western Blot: Approx 20-22 kDa band observed in Human Cerebellum and Frontal Cortex Brain lysates (calculated MW of 22.4 kDa according to NP_742011.1) Recommended concentration: 0.03-0.1 μg/mL. Primary incubation 1 hour at room temperature.
Peptide ELISA: antibody detection limit dilution 1:64000.
- Comment
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Immunofluorescence: Strong expression of the protein seen in the cytoplasm and membrane of HeLa cells. Recommended concentration: 10μg/ml.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Minimize freezing and thawing.
- Storage
- -20 °C
- Storage Comment
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- Target
- TNFSF14 (Tumor Necrosis Factor (Ligand) Superfamily, Member 14 (TNFSF14))
- Alternative Name
- TNFSF14 (TNFSF14 Products)
- Synonyms
- TNFSF14 antibody, CD258 antibody, HVEML antibody, LIGHT antibody, LTg antibody, TR2 antibody, HVEM-L antibody, Ly113 antibody, TNF superfamily member 14 antibody, tumor necrosis factor (ligand) superfamily, member 14 antibody, TNFSF14 antibody, Tnfsf14 antibody
- Background
- TNFSF14, tumor necrosis factor (ligand) superfamily, member 14, CD258, HVEML, LIGHT, LTg, TR2, delta transmembrane LIGHT, herpesvirus entry mediator A, ligand for herpesvirus entry mediator, tumor necrosis factor ligand superfamily, member 14, tumor necro
- Gene ID
- 8740
- NCBI Accession
- NP_003798, NP_742011
- Pathways
- Cancer Immune Checkpoints
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