NFAT1 antibody (Internal Region)
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- Target See all NFAT1 Antibodies
- NFAT1 (Nuclear Factor of Activated T-Cells, Cytoplasmic, Calcineurin-Dependent 2 (NFAT1))
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Binding Specificity
- Internal Region
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Reactivity
- Human
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- This NFAT1 antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
- Purpose
- NFATC2 / NFAT1
- Sequence
- QQFRTGLSSP DARYQ
- Specificity
- This antibody is expected to recognize all three reported isoforms (NP_036472.2, NP_775114.1, NP_001129493.1).
- Cross-Reactivity
- Cow, Dog, Human, Mouse, Rat
- Purification
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Grade
- Verified
- Immunogen
- Peptide with sequence C-QQFRTGLSSPDARYQ, from the internal region of the protein sequence according to NP_036472.2, NP_775114.1, NP_001129493.1.
- Isotype
- IgG
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- Application Notes
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Immunohistochemistry: In paraffin embedded Human Spleen shows nuclear membrane and weaker cytoplasm staining in select splenocytes. Recommended concentration, 3-6 μg/mL.
Western Blot: Approx 110 kDa band observed in Human lysates of cell line MOLT4 (calculated MW of 100 kDa according to NP_775114.1). Recommended concentration: 0.3-1 μg/mL.
Peptide ELISA: antibody detection limit dilution 1:64000.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Minimize freezing and thawing.
- Storage
- -20 °C
- Storage Comment
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- Target
- NFAT1 (Nuclear Factor of Activated T-Cells, Cytoplasmic, Calcineurin-Dependent 2 (NFAT1))
- Alternative Name
- NFATC2 (NFAT1 Products)
- Synonyms
- NFATC2 antibody, AI607462 antibody, NF-ATc2 antibody, NFAT1 antibody, NFAT1-D antibody, Nfatp antibody, NFATP antibody, nuclear factor of activated T-cells 2 antibody, nuclear factor of activated T cells, cytoplasmic, calcineurin dependent 2 antibody, NFATC2 antibody, nfatc2 antibody, Nfatc2 antibody
- Background
- NFATC2, nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 2, KIAA0611, NFAT1, NFATP, NFAT pre-existing subunit, NFAT transcription complex, preexisting component, OTTHUMP00000031291, T cell transcription factor NFAT1, nuclear factor
- Gene ID
- 4773, 140488, 312320
- NCBI Accession
- NP_036472, NP_775114, NP_001129493
- Pathways
- RTK Signaling, WNT Signaling, Fc-epsilon Receptor Signaling Pathway, VEGF Signaling, BCR Signaling
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