ACPP antibody (Internal Region)

Catalog No. ABIN570986

Product Details anti-ACPP Antibody

Target: ACPP (Acid Phosphatase, Prostate (ACPP))

Binding Specificity: Internal Region

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This ACPP antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Purpose: ACPP / PAP

Sequence: YGIHKQKEKS RLQ

Specificity: This antibody is expected to recognize both isoforms (NP_001090.2, NP_001127666.1).

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: C-YGIHKQKEKSRLQ

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Prostate shows cytoplasm staining in some secretory cells of the gland. Recommended concentration, 3-5 μg/mL. This antibody has been successfully used in IHC on Mouse: Nousiainen HO et al. (2014) PMID: 2484

Western Blot: Approx 50 kDa band observed in Human Prostate lysates (calculated MW of 44.6 kDa according to NP_001090.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Patel et al, ,Clin Chem. 1986 O

Peptide ELISA: antibody detection limit dilution 1:64000.

Comment:

Immunofluorescence:: This antibody has been successfully used in IF on Mouse: Nousiainen HO et al. (2014) PMID: 24846136.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-ACPP antibody (ABIN570986)

Nousiainen, Quintero, Myöhänen, Voikar, Mijatovic, Segerstråle, Herrala, Kulesskaya, Pulkka, Kivinummi, Abo-Ramadan, Taira, Piepponen, Rauvala, Vihko: "Mice deficient in transmembrane prostatic acid phosphatase display increased GABAergic transmission and neurological alterations." in: PLoS ONE, Vol. 9, Issue 5, pp. e97851, (2014) (PubMed).

Target Details for ACPP

Target: ACPP (Acid Phosphatase, Prostate (ACPP))

Alternative Name: ACP3 (ACPP Products)

Synonyms: 5'-NT antibody, ACP-3 antibody, ACP3 antibody, PAP antibody, AMAP2 antibody, CENTB3 antibody, DDEF2 antibody, PAG3 antibody, Pap-alpha antibody, SHAG1 antibody, A030005E02Rik antibody, FRAP antibody, Lap antibody, Ppal antibody, Acpp11 antibody, RNACPP11 antibody, pap antibody, ACPP antibody, acp-3 antibody, acp3 antibody, acpt antibody, acid phosphatase, prostate antibody, ArfGAP with SH3 domain, ankyrin repeat and PH domain 2 antibody, prostatic acid phosphatase antibody, prostatic acid phosphatase, putative antibody, acid phosphatase, prostate S homeolog antibody, ACPP antibody, ASAP2 antibody, Acpp antibody, CpipJ_CPIJ004002 antibody, Smp_016640 antibody, acpp.S antibody

Background: ACPP, acid phosphatase, prostate, ACP-3, ACP3, PAP, prostatic acid phosphotase

Gene ID: 55, 56318, 56780

NCBI Accession: NP_001090

Pathways: Synaptic Membrane, Ribonucleoside Biosynthetic Process