Unc5c antibody (Internal Region)

Catalog No. ABIN570965

Product Details anti-Unc5c Antibody

Target: Unc5c (Unc-5 Homolog C (C. Elegans) (Unc5c))

Binding Specificity: Internal Region

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This Unc5c antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Purpose: UNC5C

Sequence: NCTVSEEPTG ID

Cross-Reactivity: Dog, Human, Mouse

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence NCTVSEEPTGID, from the internal region of the protein sequence according to NP_003719.2.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Cerebral Cortex shows staining of some neuronal bodies plus intense axonal staining. Recommended concentration: 3-5 μg/mL.

Western Blot: Preliminary experiments in Human and Mouse Brain lysates gave no specific signal but low background (at antibody concentration up to 1 μg/mL).

Peptide ELISA: antibody detection limit dilution 1:32000.

Comment:

Immunofluorescence: Staining of paraffin embedded endocrine islets (red) in adult Mouse Pancreas. Nuclear staining in blue and ductal cell staining in green. Data obtained from a customer.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for Unc5c

Target: Unc5c (Unc-5 Homolog C (C. Elegans) (Unc5c))

Alternative Name: UNC5C (Unc5c Products)

Background: UNC5C, unc-5 homolog C (C. elegans), UNC5H3, homolog of C. elegans transmembrane receptor Unc5, unc5 (C.elegans homolog) c, unc5C

Gene ID: 8633, 22253

NCBI Accession: NP_003719