ITM2B antibody (Internal Region)

Catalog No. ABIN570955

Product Details anti-ITM2B Antibody

Target: ITM2B (Integral Membrane Protein 2B (ITM2B))

Binding Specificity: Internal Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This ITM2B antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

Purpose: ITM2B

Sequence: HDKETYKLQR RETIK

Cross-Reactivity: Cow, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-HDKETYKLQRRETIK, from the internal region of the protein sequence according to NP_068839.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Small Intestine. Recommended concentration: 2.5 μg/mL.

Western Blot: Approx. 30-35 kDa band observed in HepG2 cell lysates (calculated MW of 30.3 kDa according to NP_068839.1). Recommended concentration: 0.1-0.3 μg/mL. Primary incubation 1 hour at room temperature. Preliminary testing was unsuccessful on Rat an

Peptide ELISA: antibody detection limit dilution 1:8000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm and Golgi apparatus of A431 and HeLa cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recomme

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for ITM2B

Target: ITM2B (Integral Membrane Protein 2B (ITM2B))

Alternative Name: ITM2B (ITM2B Products)

Background: ITM2B, integral membrane protein 2B, ABRI, BRI, BRI2, BRICD2B, E25B, E3-16, FBD, BRICHOS domain containing 2B, OTTHUMP00000018394

Gene ID: 9445, 16432, 290364

NCBI Accession: NP_068839