DIAPH1 antibody (Internal Region)

Catalog No. ABIN570942

Product Details anti-DIAPH1 Antibody

Target: DIAPH1 (Diaphanous Homolog 1 (DIAPH1))

Binding Specificity: Internal Region

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This DIAPH1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purpose: DIAPH1

Sequence: QTKTSKAKKD Q

Specificity: This antibody is expected to recognize both reported isoforms (NP_005210.3, NP_001073280.1).

Cross-Reactivity: Human, Mouse

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-QTKTSKAKKDQ, from the internal region of the protein sequence according to NP_005210.3, NP_001073280.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 140 kDa band observed in lysates of cell line NIH3T3 (calculated MW of 141 kDa according to Human NP_005210.3 and 139 kDa according to Mouse NP_031884.1). Recommended concentration: 0.3-1 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for DIAPH1

Target: DIAPH1 (Diaphanous Homolog 1 (DIAPH1))

Alternative Name: DIAPH1 (DIAPH1 Products)

Background: DIAPH1, diaphanous homolog 1 (Drosophila), DFNA1, DIA1, DRF1, FLJ25265, LFHL1, hDIA1, diaphanous 1, diaphanous-1, diaphanous-related formin 1

Gene ID: 1729, 13367

NCBI Accession: NP_005210, NP_001073280

Pathways: Sensory Perception of Sound