Septin 6 antibody (Internal Region)

Catalog No. ABIN570940

Product Details anti-Septin 6 Antibody

Target: Septin 6 (SEPT6)

Binding Specificity: Internal Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Septin 6 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Purpose: SEPT6

Sequence: DEVNAFKQRK TA

Specificity: This antibody is expected to recognize the reported isoforms NP_665798.1, NP_055944.2 and NP_665801.1. Reported variants NP_665799.1 and NP_665798.1 represent identical protein.

Cross-Reactivity: Cow, Dog, Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-DEVNAFKQRKTA, from the internal region of the protein sequence according to NP_665798.1, NP_055944.2, NP_665801.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Tonsil shows specific staining in dividing cells just outside a germinal centre. Recommended concentration, 3-5 μg/mL.

Western Blot: Approx 50 kDa band observed in Human Testis, Duodenum, Kidney and Tonsil lysates, and in lysates of cell lines Daudi, Jurkat and MOLT4 (calculated MW of 49.7 kDa according to NP_055944.2). Recommended concentration: 0.1-1 μg/mL. Primary incuba

Peptide ELISA: antibody detection limit dilution 1:4000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for Septin 6

Target: Septin 6 (SEPT6)

Alternative Name: SEPTIN6 (SEPT6 Products)

Background: SEPT6, septin 6, KIAA0128, MGC16619, MGC20339, SEP2, SEPT2, septin 2

Gene ID: 23157

NCBI Accession: NP_665798, NP_055944, NP_665801