LIMS2 antibody (Internal Region)

Catalog No. ABIN570889

Product Details anti-LIMS2 Antibody

Target: LIMS2 (LIM and Senescent Cell Antigen-Like Domains 2 (LIMS2))

Binding Specificity: Internal Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This LIMS2 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC)

Purpose: LIMS2

Sequence: RWSNMSDALA

Specificity: This antibody is expected to recognize isoform 1 (NP_001129509.2) and isoformn 3 (NP_001154875.1).

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: C-RWSNMSDALA

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Spleen. Recommended concentration: 3.75 μg/mL.

Western Blot: Preliminary experiments gave an approx 80 kDa band in Human Heart and Mouse Heart lysates after 0.5 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calcula

Peptide ELISA: antibody detection limit dilution 1:8000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for LIMS2

Target: LIMS2 (LIM and Senescent Cell Antigen-Like Domains 2 (LIMS2))

Alternative Name: LIMS2 (LIMS2 Products)

Synonyms: PINCH2 antibody, PINCH-2 antibody, LIMS1 antibody, LIM and senescent cell antigen like domains 2 antibody, LIM zinc finger domain containing 2 antibody, Lims2 antibody, LIMS2 antibody

Background: LIMS2, LIM and senescent cell antigen-like domains 2, FLJ10044, PINCH2, ILK-binding protein, OTTHUMP00000203848, OTTHUMP00000203850, OTTHUMP00000203866

Gene ID: 55679

NCBI Accession: NP_001129509, NP_001154875

Pathways: Cell-Cell Junction Organization