Desmoplakin antibody (Internal Region)

Catalog No. ABIN570871

Product Details anti-Desmoplakin Antibody

Target: Desmoplakin (DSP)

Binding Specificity: Internal Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Desmoplakin antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA

Purpose: Desmoplakin (aa 1672 -1684)

Sequence: QESVKQAHLR NEH

Specificity: This antibody is expected to recognize reported isoform I (NP_004406.2) only.

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: C-QESVKQAHLRNEH

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Heart shows staining of the junctons between cardiomuscular fibres. Recommended concentration, 3-6 μg/mL.

Western Blot: Preliminary experiments in lysates of cell lines HEK293, HeLa and NIH3T3 gave no specific signal but low background (at antibody concentration up to 1 μg/mL). We would appreciate any feedback from people in the field - have any results been

Peptide ELISA: antibody detection limit dilution 1:4000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for Desmoplakin

Target: Desmoplakin (DSP)

Alternative Name: DSP (DSP Products)

Synonyms: DP antibody, DPI antibody, DPII antibody, DSP antibody, dpi antibody, dpii antibody, 2300002E22Rik antibody, 5730453H04Rik antibody, AA407887 antibody, AA407888 antibody, AW109828 antibody, im:6911953 antibody, sb:cb570 antibody, wu:fc17a08 antibody, wu:fk73d01 antibody, desmoplakin antibody, Desmoplakin antibody, ORF113 antibody, desmoplakin a antibody, ORF112 DESMOPLAKIN antibody, orf102 antibody, orf91; similar to Cydia pomonella granulovirus orf112 antibody, ORF62 antibody, ORF-66 antibody, ORF57 antibody, agip105 antibody, similar to XcORF133; similar to HaGV desmoplakin antibody, desmoplakin L homeolog antibody, ORF96 antibody, DSP antibody, PhopGV104 antibody, ORF-59 desmoplakin antibody, desmop antibody, Dsp antibody, dsp antibody, desmoplakin antibody, dspa antibody, orf112 desmoplakin antibody, ClgVgp102 antibody, COGV_gp091 antibody, APNV_p087 antibody, CBNV_gp066 antibody, EONV_gp057 antibody, OLNV_gp066 antibody, AgipMNPV_gp105 antibody, HaMNV_gp106 antibody, EupsNPV_gp065 antibody, PsunGV_gp139 antibody, dsp.L antibody, ClanGV_gp096 antibody

Background: DSP, desmoplakin, DPI, 250 kDa paraneoplastic pemphigus antigen, desmoplakin I

Gene ID: 1832, 109620, 306871

NCBI Accession: NP_004406

Pathways: Cell-Cell Junction Organization