IDE antibody

Catalog No. ABIN5708390

Product Details anti-IDE Antibody

Target: IDE (Insulin-Degrading Enzyme (IDE))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IDE antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)

Purification: Antigen affinity purified

Immunogen: A recombinant human protein corresponding to amino acids F485-K756 was used as the immunogen for the IDE antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the IDE antibody should be determined by the researcher.\. Western blot: 0.1-0.5 μg/mL, IHC (FFPE): 0.5-1 μg/mL,FACS: 1-3 μg/10^6 cells, Direct ELISA: 0.1-0.5 μg/mL

Restrictions: For Research Use only

Handling

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: -20 °C

Storage Comment: After reconstitution, the IDE antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Target Details for IDE

Target: IDE (Insulin-Degrading Enzyme (IDE))

Alternative Name: IDE / Insulin degrading enzyme (IDE Products)

Background: Insulin-degrading enzyme, also known as IDE, is an enzyme. This gene encodes a zinc metallopeptidase that degrades intracellular insulin, and thereby terminates insulins activity, as well as participating in intercellular peptide signalling by degrading diverse peptides such as glucagon, amylin, bradykinin, and kallidin. The preferential affinity of this enzyme for insulin results in insulin-mediated inhibition of the degradation of other peptides such as beta-amyloid. Deficiencies in this protein's function are associated with Alzheimer's disease and type 2 diabetes mellitus but mutations in this gene have not been shown to be causitive for these diseases. This protein localizes primarily to the cytoplasm but in some cell types localizes to the extracellular space, cell membrane, peroxisome, and mitochondrion. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

UniProt: P14735

Pathways: SARS-CoV-2 Protein Interactome