MAML3 antibody

Catalog No. ABIN5707997

Product Details anti-MAML3 Antibody

Target: MAML3 (Mastermind-Like 3 (MAML3))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MAML3 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: Purified

Purity: Protein G affinity chromatography

Immunogen: A human full length recombinant protein was used as the immunogen for this MAML3 antibody.

Clone: MAML3-1303

Isotype: IgG1

Application Details

Application Notes: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the MAML3 antibody to be titered up or down for optimal performance.\. FACS: 0.5-1 μg/10^6 cells,Immunofluorescence: 1-2 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL

Restrictions: For Research Use only

Handling

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the MAML3 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details for MAML3

Target: MAML3 (Mastermind-Like 3 (MAML3))

Alternative Name: MAML3 (MAML3 Products)

Synonyms: AV234550 antibody, BC049812 antibody, Mam-2 antibody, mKIAA1816 antibody, Glrp1 antibody, CAGH3 antibody, ERDA3 antibody, GDN antibody, MAM-2 antibody, MAM2 antibody, TNRC3 antibody, mastermind like 3 (Drosophila) antibody, mastermind-like transcriptional coactivator 3 antibody, mastermind like transcriptional coactivator 3 antibody, Maml3 antibody, MAML3 antibody

Background: Mastermind-like 3 is a nuclear speckle protein that acts as a transcriptional coactivator for Notch receptors. The Notch signaling pathway influences cell fate by regulating the ability of precursor cells to properly respond to developmental signals. MAML3 is a member of the mastermind-like family of proteins that are human homologs of the Drosophila melanogaster mastermind protein. Through its N-terminal region, MAML3 interacts with the ankyrin repeats of the Notch 1-4 proteins. This interaction leads to formation of a DNA-binding complex with the Notch proteins and RBP-Jκ, a complex that can then induce HES1 gene expression. While the N-terminal domain of MAML3 is essential for proper Notch binding, the C-terminal domain is essential for transcriptional activation. Due to its involvement in cell signaling and transcriptional activation, its upregulation is thought to be involved in oncogenesis.