UBR4 antibody (C-Term)

Catalog No. ABIN570777

Product Details anti-UBR4 Antibody

Target: UBR4 (Ubiquitin Protein Ligase E3 Component N-Recognin 4 (UBR4))

Binding Specificity: C-Term

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This UBR4 antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA

Purpose: UBR4 / ZUBR1

Sequence: NTTRATSREE KNLQ

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-NTTRATSREEKNLQ, from the C Terminus of the protein sequence according to NP_065816.2.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Spleen shows various types of subnuclear staining in select splenocytes and additional membrane plus cytoplasm staining elsewhere. Recommended concentration, 5-10 μg/mL.

Western Blot: Not yet tested - our routinely used western blotting protocol does not allow detection of proteins as large as the calculated size of 574 kDa according to NP_065816.2. Therefore we cannot recommend an optimal concentration and the antibody i

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for UBR4

Target: UBR4 (Ubiquitin Protein Ligase E3 Component N-Recognin 4 (UBR4))

Alternative Name: UBR4 (UBR4 Products)

Background: UBR4, ubiquitin protein ligase E3 component n-recognin 4, FLJ41863, KIAA0462, KIAA1307, RBAF600, ZUBR1, p600, retinoblastoma-associated factor 600, retinoblastoma-associated factor 600-like protein, zinc finger, UBR1 type 1

Gene ID: 23352, 69116, 313658

NCBI Accession: NP_065816