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SMC5 antibody (Internal Region)

Verified SMC5 Reactivity: Human ELISA, IF, FACS Host: Goat Polyclonal unconjugated
Catalog No. ABIN570758
  • Target See all SMC5 Antibodies
    SMC5 (Structural Maintenance of Chromosomes 5 (SMC5))
    Binding Specificity
    • 15
    • 5
    • 3
    • 1
    • 1
    Internal Region
    Reactivity
    • 34
    • 18
    • 11
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Host
    • 33
    • 1
    Goat
    Clonality
    • 34
    Polyclonal
    Conjugate
    • 9
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This SMC5 antibody is un-conjugated
    Application
    • 23
    • 13
    • 13
    • 10
    • 3
    • 3
    • 2
    • 2
    • 2
    ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)
    Purpose
    SMC5
    Sequence
    TSKHLEHKDN ELRQ
    Cross-Reactivity
    Cow, Dog, Human, Mouse, Rat
    Purification
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    Grade
    Verified
    Immunogen
    Peptide with sequence C-TSKHLEHKDNELRQ, from the Internal region of the protein sequence according to NP_055925.2.
    Isotype
    IgG
  • Application Notes

    Western Blot: Preliminary testing showed bands at approx 100+150 kDa in HEK293 nuclear lysates after 1 μg/mL antibody staining (calculated MW of 129 kDa according to NP_055925.2). These bands were successfully blocked by incubation with the immunizing pept

    Peptide ELISA: antibody detection limit dilution 1:128000.

    Comment

    Immunofluorescence: Strong expression of the protein seen in HeLa cells. Recommended concentration: 10μg/ml.

    Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.5 mg/mL
    Buffer
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Minimize freezing and thawing.
    Storage
    -20 °C
    Storage Comment
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Target
    SMC5 (Structural Maintenance of Chromosomes 5 (SMC5))
    Alternative Name
    SMC5 (SMC5 Products)
    Synonyms
    F1N13.60 antibody, F1N13_60 antibody, structural maintenance of chromosomes 5 antibody, SMC-5 antibody, SMC5L1 antibody, MLZ-453 antibody, Smc5l1 antibody, mKIAA0594 antibody, mSMC5 antibody, structural maintenance of chromosomes 5 antibody, structural maintenance of chromosomes 5 S homeolog antibody, SMC5 antibody, smc5 antibody, Smc5 antibody, smc5.S antibody
    Background
    SMC5, structural maintenance of chromosomes 5, KIAA0594, SMC5L1, OTTHUMP00000063367, SMC5 protein, SMC5 structural maintenance of chromosomes 5-like 1
    Gene ID
    23137, 226026, 293967
    NCBI Accession
    NP_055925
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