FOXP3 antibody (FITC)

Catalog No. ABIN5707149

Product Details anti-FOXP3 Antibody (FITC)

Target: FOXP3 (Forkhead Box P3 (FOXP3))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This FOXP3 antibody is conjugated to FITC

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: Human Foxp3 (Mr ~57 kDa). Does not cross react with Mouse Foxp3. T regulatory cells (Originally called T suppressor cells) are a subpopulation of T cells. Their major roles are to suppress excess immune response of other cells and to maintain tolerance of self-antigens. Initially, researchers used CD4 and CD25 (IL-2 receptor) to identify T suppress subpopulation and purified CD4+CD25+ T cells were used for T cells Suppression Assay, however some other activated T cells also express CD25.1,2 The most convincible molecular marker of Treg cells is Forkhead box protein 3 (Foxp3) which is expressed in the nucleus.3 Anti-Foxp3 antibodies are now widely applied to identify the roles of Treg cells in the development of many diseases, such as cancer, autoimmune disease, and organ transplantation.4,5,6 Scientists found that the over-activation of Treg cells may cause cancer cells to escape from immune surveillance 5. Conversely, patients with autoimmune diseases such as psoriasis, systemic lupus erythematous (SLE) have relative dysfunction of Foxp3 positive cells.7,8 Immunologists are trying to induce immune tolerance in organ transplantation using Treg cells.6

Characteristics: Mouse Anti-Human Foxp3-FITC

Immunogen: Full length recombinant Human Foxp3

Clone: SB151b

Isotype: IgG1

Application Details

Application Notes:

• Applications: Flow cytometry Immunohistochemistry (frozen sections) Western blotting
• Working Dilutions: Flow Cytometry: FITC conjugate 10 L/106 cells AF647 conjugate 10 L/106 cells Western Blotting: Purified antibody 1-5 g/mL

Restrictions: For Research Use only

Handling

Buffer: 100 Tests in 1.0 mL PBS/Sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2-8°C

Target Details for FOXP3

Target: FOXP3 (Forkhead Box P3 (FOXP3))

Alternative Name: Foxp3 (FOXP3 Products)

Synonyms: foxp3 antibody, FOXP3 antibody, AIID antibody, DIETER antibody, IPEX antibody, PIDX antibody, XPID antibody, JM2 antibody, scurfin antibody, sf antibody, RGD1562112 antibody, forkhead box P3 antibody, forkhead box P3a antibody, forkhead box P3 L homeolog antibody, FOXP3 antibody, foxp3a antibody, foxp3 antibody, foxp3.L antibody, Foxp3 antibody

Pathways: Chromatin Binding, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation