CEP290 antibody (Internal Region)

Catalog No. ABIN570714

Product Details anti-CEP290 Antibody

Target: CEP290 (Centrosomal Protein 290kDa (CEP290))

Binding Specificity: Internal Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This CEP290 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Purpose: CEP290

Sequence: RNSKHLKQQQ YRAEN

Specificity: Despite similarity of the immunizing peptide with the mouse protein, this antibody was demonstrated to weak for mouse Cep290.

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-RNSKHLKQQQYRAEN , from the internal region of the protein sequence according to NP_079390.3.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Prostate. Recommended concentration: 5-7 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:128000.

Comment:

Immunocytochemistry: Formaldehyde-fixed cell lines derived from Prostate Cancer and from Breast Cancer show typical centrosomal staining. Recommended concentration: 2-3ug/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for CEP290

Target: CEP290 (Centrosomal Protein 290kDa (CEP290))

Alternative Name: CEP290 (CEP290 Products)

Background: CEP290, centrosomal protein 290 kDa, 3H11Ag, BBS14, FLJ13615, FLJ21979, JBTS5, JBTS6, KIAA0373, LCA10, MKS4, NPHP6, SLSN6, rd16, CTCL tumor antigen se2-2, monoclonal antibody 3H11 antigen, nephrocytsin-6, prostate cancer antigen T21

Gene ID: 80184, 216274

NCBI Accession: NP_079390

Pathways: cAMP Metabolic Process, M Phase