SHMT1 antibody

Catalog No. ABIN5706931

Product Details anti-SHMT1 Antibody

Target: SHMT1 (serine Hydroxymethyltransferase 1 (Soluble) (SHMT1))

Reactivity: Vicia faba, Spinach, Pisum sativum, Arabidopsis thaliana

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SHMT1 antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: The antibody is very specific for salmonid inducible form - hsp70.

Cross-Reactivity (Details): Not reactive in: no confirmed exceptions from predicted reactivity known in the moment

Predicted Reactivity: dicots including: Glycine max, Medicago truncatula, Ricinus communis, Solanum tuberosum, Vitis vinifera, monocots: Hordeum vulgare, trees: Populus balsamifera, algae: Chlamydomonas reinhardii

Characteristics: Expected / apparent Molecular Weight of the Antigene: 53 kDa (Arabidopsis thaliana)

Purification: affinity purified

Immunogen: Purified SHMT protein from Spinacia oleracea

Isotype: IgG

Application Details

Application Notes: 1:5 000 with standard ECL (WB)

Comment:

this antibody can be used on total leaf extracts and isolated mitochondria

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 200 μL of sterile water.

Buffer: PBS pH 7.4

Handling Advice: Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

References for anti-SHMT1 antibody (ABIN5706931)

Wei, Sun, Sandoval, Cross, Gordon, Kang, Roje: "Folate polyglutamylation eliminates dependence of activity on enzyme concentration in mitochondrial serine hydroxymethyltransferases from Arabidopsis thaliana." in: Archives of biochemistry and biophysics, Vol. 536, Issue 1, pp. 87-96, (2013) (PubMed).

Target Details for SHMT1

Target: SHMT1 (serine Hydroxymethyltransferase 1 (Soluble) (SHMT1))

Alternative Name: SHMT (SHMT1 Products)

Synonyms: Shmt antibody, mShmt antibody, LRRGT00032 antibody, shmt1 antibody, MGC53442 antibody, zgc:66171 antibody, zgc:77524 antibody, SHMT1 antibody, Shmt1 antibody, CSHMT antibody, SHMT antibody, AI324848 antibody, AI385541 antibody, C81125 antibody, mshmt antibody, mshmt1 antibody, mshmt2 antibody, MEL-32 antibody, SHMT2 antibody, F20D10.50 antibody, F20D10_50 antibody, SERINE HYDROXYMETHYLTRANSFERASE 1 antibody, SERINE TRANSHYDROXYMETHYLASE antibody, SERINE TRANSHYDROXYMETHYLTRANSFERASE antibody, STM antibody, serine transhydroxymethyltransferase 1 antibody, serine hydroxymethyltransferase 1 antibody, serine hydroxymethyltransferase 1 (soluble) L homeolog antibody, serine hydroxymethyltransferase 1 (soluble) antibody, microRNA 6778 antibody, serine transhydroxymethyltransferase 1 antibody, Shmt1 antibody, shmt1.L antibody, shmt1 antibody, MIR6778 antibody, SHMT1 antibody, SHM1 antibody

Background: Serine hydroxymethyltransferase (SHMT) is part of the mitochondrial enzyme complex.

Molecular Weight: 53 kDa (Arabidopsis thaliana)