Luciferase antibody
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- Target See all Luciferase Antibodies
- Luciferase
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Reactivity
- Vibrio fischeri
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This Luciferase antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Dot Blot (DB)
- Supplier Product No.
- 100-401-137-0100
- Supplier
- Rockland
- Purpose
- Luciferase Antibody
- Cross-Reactivity (Details)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, purified and partially purified Luciferase [Photobacterium fischerii].
- Characteristics
- Synonyms: rabbit anti-Luciferase Antibody, Alkanal monooxygenase alpha chain, Bacterial luciferase alpha chain, luxA, Alkanal monooxygenase beta chain, Bacterial luciferase beta chain, luxB
- Purification
- This product was prepared from monospecific antiserum by a delipidation and defibrination.
- Immunogen
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Immunogen: Luciferase [Photobacterium fischerii]
Immunogen Type: Native Protein
- Top Product
- Discover our top product Luciferase Primary Antibody
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- Application Notes
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Immunohistochemistry Dilution: 1:500 - 1:2,500
Application Note: Luciferase Antibody has been tested by dot blot and is suitable for western blotting, immunohistochemistry, immunoprecipitation, and ELISA. Researchers should determine optimal titers for applications that are not stated below.
Western Blot Dilution: 1:1,000 - 1:5,000
Immunoprecipitation Dilution: 1:100
ELISA Dilution: 1:5,000 - 1:25,000
Other: User Optimized
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
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Reconstitution Volume: 2.0 mL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- Concentration
- 75 mg/mL
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide - Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- Expiry Date
- 12 months
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Increased expression of the urokinase plasminogen activator system by Helicobacter pylori in gastric epithelial cells." in: American journal of physiology. Gastrointestinal and liver physiology, Vol. 295, Issue 3, pp. G431-41, (2008) (PubMed).
: "
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Increased expression of the urokinase plasminogen activator system by Helicobacter pylori in gastric epithelial cells." in: American journal of physiology. Gastrointestinal and liver physiology, Vol. 295, Issue 3, pp. G431-41, (2008) (PubMed).
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- Target
- Luciferase
- Abstract
- Luciferase Products
- Background
- Background: Anti luciferase Antibody recognizes luciferase that is commonly used in biological research as a reporter to assess the transcriptional activity in cells that are transfected with a genetic construct containing the luciferase gene under the control of a promoter of interest. Luciferase can also be used to detect the level of cellular ATP in cell viability assays or for kinase activity assays. Additionally proluminescent molecules that are converted to luciferin upon activity of a particular enzyme can be used to detect enzyme activity in coupled or two-step luciferase assays. Such substrates have been used to detect caspase activity and cytochrome P450 activity, among others.
- Gene ID
- 3280269
- UniProt
- P19907
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