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F11R antibody (pTyr280)

Rockland 600-401-gn5 F11R Reactivity: Human WB, ELISA, IF, FM Host: Rabbit Polyclonal unconjugated
Rockland
Catalog No. ABIN5706868
Supplier Product No.: 600-401-gn5
  • Target See all F11R Antibodies
    F11R (F11 Receptor (F11R))
    Binding Specificity
    • 12
    • 5
    • 5
    • 5
    • 4
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    pTyr280
    Reactivity
    • 71
    • 45
    • 35
    • 9
    • 6
    • 6
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    Human
    Host
    • 62
    • 16
    • 14
    • 1
    Rabbit
    Clonality
    • 53
    • 36
    • 2
    Polyclonal
    Conjugate
    • 46
    • 8
    • 7
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This F11R antibody is un-conjugated
    Application
    • 59
    • 28
    • 22
    • 16
    • 16
    • 14
    • 7
    • 7
    • 3
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunofluorescence (IF), Fluorescence Microscopy (FM)
    Supplier Product No.
    600-401-gn5
    Supplier
    Rockland
    Purpose
    JAM A phospho Y280 Antibody
    Cross-Reactivity (Details)
    Anti-JAM A pY280 is directed against human JAM A phosphorylated at the tyrosine 280 position.
    Characteristics
    Synonyms: rabbit anti-JAM A pY280 antibody, JAM-A, Junctional adhesion molecule A, JAM-1, Junctional adhesion molecule 1, Platelet F11 receptor, Platelet adhesion molecule 1, PAM-1, CD321, JAM1, JCAM, JAM 1, JAMA
    Purification
    This product is an affinity purified antibody produced by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide to remove any unwanted reactivities.
    Sterility
    Sterile filtered
    Immunogen

    Immunogen: Affinity purified Anti-JAM A pY280 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the c-term and phosphorylated at the tyrosine 280 position of Human JAM A protein.

    Immunogen Type: Conjugated Peptide

    Isotype
    IgG
    Top Product
    Discover our top product F11R Primary Antibody
  • Application Notes

    Application Note: This affinity purified antibody has been tested for use in ELISA, IF, and western blot.   Specific conditions for reactivity should be optimized by the end user. Expect a band ~ 32.5 kDa in size corresponding to JAM A by western blotting in the appropriate cell lysate or extract.

    Western Blot Dilution: 1 μg/mL

    ELISA Dilution: 5 μg/mL

    IF Microscopy Dilution: User optimized

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1.95 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Store vial at -20° C prior to opening.  Aliquot contents and freeze at -20° C or below for extended storage.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.
    Expiry Date
    12 months
  • Tapia, Kralicek, Hecht: "Enteropathogenic Escherichia coli (EPEC) Recruitment of PAR Polarity Protein Atypical PKCζ to Pedestals and Cell-Cell Contacts Precedes Disruption of Tight Junctions in Intestinal Epithelial Cells." in: International journal of molecular sciences, Vol. 21, Issue 2, (2020) (PubMed).

    Fan, Weight, Luissint, Hilgarth, Brazil, Ettel, Nusrat, Parkos: "Role of JAM-A tyrosine phosphorylation in epithelial barrier dysfunction during intestinal inflammation." in: Molecular biology of the cell, Vol. 30, Issue 5, pp. 566-578, (2019) (PubMed).

  • Target
    F11R (F11 Receptor (F11R))
    Alternative Name
    F11R (F11R Products)
    Background
    Background: Anti-JAM A pY280 was designed, produced, and validated as part of the Joy Cappel Young Investigator Award (JCYIA). JAM A pY280 Antibody detects junctional adhesion molecule A (JAM A) protein phosphorylated at the tyrosine 280 location. JAM A is involved in cell-cell contact and tight junction formation in epithelial cells. JAM A protein is a transmembrane protein expressed in epithelial and endothelial cells as well as in hematopoietic cells and is important for a variety of cellular processes including tight junction assembly, leukocyte transmigration, platelet activation, angiogenesis and virus binding. In addition, JAM A may be a potential therapeutic target for breast cancer.
    Gene ID
    50848
    NCBI Accession
    NP_058642
    Pathways
    Cell-Cell Junction Organization
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