F11R antibody (pTyr280)
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- Target See all F11R Antibodies
- F11R (F11 Receptor (F11R))
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Binding Specificity
- pTyr280
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This F11R antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Immunofluorescence (IF), Fluorescence Microscopy (FM)
- Supplier Product No.
- 600-401-gn5
- Supplier
- Rockland
- Purpose
- JAM A phospho Y280 Antibody
- Cross-Reactivity (Details)
- Anti-JAM A pY280 is directed against human JAM A phosphorylated at the tyrosine 280 position.
- Characteristics
- Synonyms: rabbit anti-JAM A pY280 antibody, JAM-A, Junctional adhesion molecule A, JAM-1, Junctional adhesion molecule 1, Platelet F11 receptor, Platelet adhesion molecule 1, PAM-1, CD321, JAM1, JCAM, JAM 1, JAMA
- Purification
- This product is an affinity purified antibody produced by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide to remove any unwanted reactivities.
- Sterility
- Sterile filtered
- Immunogen
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Immunogen: Affinity purified Anti-JAM A pY280 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the c-term and phosphorylated at the tyrosine 280 position of Human JAM A protein.
Immunogen Type: Conjugated Peptide
- Isotype
- IgG
- Top Product
- Discover our top product F11R Primary Antibody
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- Application Notes
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Application Note: This affinity purified antibody has been tested for use in ELISA, IF, and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band ~ 32.5 kDa in size corresponding to JAM A by western blotting in the appropriate cell lysate or extract.
Western Blot Dilution: 1 μg/mL
ELISA Dilution: 5 μg/mL
IF Microscopy Dilution: User optimized
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1.95 mg/mL
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide - Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- Expiry Date
- 12 months
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Enteropathogenic Escherichia coli (EPEC) Recruitment of PAR Polarity Protein Atypical PKCζ to Pedestals and Cell-Cell Contacts Precedes Disruption of Tight Junctions in Intestinal Epithelial Cells." in: International journal of molecular sciences, Vol. 21, Issue 2, (2020) (PubMed).
: "Role of JAM-A tyrosine phosphorylation in epithelial barrier dysfunction during intestinal inflammation." in: Molecular biology of the cell, Vol. 30, Issue 5, pp. 566-578, (2019) (PubMed).
: "
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Enteropathogenic Escherichia coli (EPEC) Recruitment of PAR Polarity Protein Atypical PKCζ to Pedestals and Cell-Cell Contacts Precedes Disruption of Tight Junctions in Intestinal Epithelial Cells." in: International journal of molecular sciences, Vol. 21, Issue 2, (2020) (PubMed).
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- Target
- F11R (F11 Receptor (F11R))
- Alternative Name
- F11R (F11R Products)
- Background
- Background: Anti-JAM A pY280 was designed, produced, and validated as part of the Joy Cappel Young Investigator Award (JCYIA). JAM A pY280 Antibody detects junctional adhesion molecule A (JAM A) protein phosphorylated at the tyrosine 280 location. JAM A is involved in cell-cell contact and tight junction formation in epithelial cells. JAM A protein is a transmembrane protein expressed in epithelial and endothelial cells as well as in hematopoietic cells and is important for a variety of cellular processes including tight junction assembly, leukocyte transmigration, platelet activation, angiogenesis and virus binding. In addition, JAM A may be a potential therapeutic target for breast cancer.
- Gene ID
- 50848
- NCBI Accession
- NP_058642
- Pathways
- Cell-Cell Junction Organization
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