ORAI1 antibody

Catalog No. ABIN5706864

Supplier Product No.: 100-401-p17

Product Details anti-ORAI1 Antibody

Target: ORAI1 (ORAI Calcium Release-Activated Calcium Modulator 1 (ORAI1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ORAI1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Supplier Product No.: 100-401-p17

Supplier: Rockland

Purpose: ORAI1 Antibody

Cross-Reactivity (Details): Anti-ORAI-1 antibody is directed against the human ORAI-1.

Characteristics: Synonyms: rabbit anti-ORAI-1 Antibody, Calcium release-activated calcium channel protein 1, orai-1, Transmembrane protein 142A, ORAI1, CRACM1, TMEM142A

Purification: The product was prepared from monospecific antiserum by delipidation and defibrination.

Sterility: Sterile filtered

Immunogen:

Immunogen: This whole rabbit serum was prepared by repeated immunizations with a recombinant fusion protein.

Immunogen Type: Recombinant Protein

Isotype: IgG

Application Details

Application Notes:

Application Note: Anti-ORAI-1 has been tested for use in western blotting. Specific conditions for reactivity should be optimized by the end user. Expect bands approximately 32.7 kDa by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1.0 μg/mL

ELISA Dilution: 1.0 μg/well

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for ORAI1

Target: ORAI1 (ORAI Calcium Release-Activated Calcium Modulator 1 (ORAI1))

Alternative Name: ORAI1 (ORAI1 Products)

Background: Background: The ORAI-1 gene codes for the calcium release-activated calcium (CRAC) channel subunit in humans. The CRAC channel subunit mediates calcium influx when activated by the calcium sensor, STIM1, following depletion of intracellular calcium stores. CRAC channels are the primary pathway for calcium influx in T-cells and promote the immune response to pathogens through activation of the transcription factor NFAT. Defects in this gene can be a cause of immune dysfunction due to inactivation of T-cells.

Gene ID: 84876

NCBI Accession: NP_116179

UniProt: Q96D31

Pathways: TCR Signaling, BCR Signaling