Fluorescein antibody

Catalog No. ABIN5706816

Supplier Product No.: 200-301-096l

Product Details

Target: Fluorescein

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

Supplier Product No.: 200-301-096l

Supplier: Rockland

Purpose: Fluorescein (FITC) Monoclonal Antibody

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-mouse serum, Fluorescein conjugated IgG and Fluorescein conjugated Bovine Serum Albumin.

Characteristics: Synonyms: mouse Anti-FITC antibody, mouse anti-fluorescein antibody

Purification: Fluorescein Antibody was prepared from mouse ascites by Protein A chromatography.

Sterility: Sterile filtered

Immunogen:

Immunogen: Fluorescein conjugated to Mouse IgG

Immunogen Type: Other

Clone: 8B9-C6-D3

Isotype: IgG2a kappa

Application Details

Application Notes:

Flow Cytometry Dilution: 1-2 μg/mL

Immunohistochemistry Dilution: 1:250 - 1:1,500

Application Note: Anti-Fluorescein Antibody has been tested by ELISA, western blot, and immunofluorescence. This product is suitable for immunomicroscopy, in situ hybridization and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.

Western Blot Dilution: 1:500 - 1:3,000

ELISA Dilution: 1:5,000 - 1:30,000

Other: in situ hybridization

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References

Kotani, Yamaguchi, Ohnishi, Kuwahara, Nakano, Nakano, Ida, Murakoshi, Honke: "Proximity proteomics identifies cancer cell membrane cis-molecular complex as a potential cancer target." in: Cancer science, Vol. 110, Issue 8, pp. 2607-2619, (2019) (PubMed).

Miyagawa-Yamaguchi, Kotani, Honke: "Expressed glycosylphosphatidylinositol-anchored horseradish peroxidase identifies co-clustering molecules in individual lipid raft domains." in: PLoS ONE, Vol. 9, Issue 3, pp. e93054, (2015) (PubMed).

Target Details

Target: Fluorescein

Target Type: Chemical

Background: Background: Anti-Fluorescein is designed for immunofluorescence microscopy, in situ hybridization, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.