Ngp antibody

Catalog No. ABIN5706813

Product Details anti-Ngp Antibody

Target: Ngp (NGP) (Neutrophilic Granule Protein (NGP))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Application: Fluorescence Microscopy (FM), Western Blotting (WB), Immunofluorescence (IF)

Purpose: Ngp Antibody

Cross-Reactivity (Details): Anti-NGP is directed against full-length mouse NGP.

Characteristics: Synonyms: rabbit anti-NGP antibody, Neutrophilic granule protein, Cystatin-like protein, Myeloid bactenecin protein, Myeloid secondary granule protein

Purification: This product is an affinity purified antibody produced by immunoaffinity chromatography using recombinant protein coupled to agarose beads.

Sterility: Sterile filtered

Immunogen:

Immunogen: Affinity purified Anti-NGP antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein of mouse NGP protein.

Immunogen Type: Recombinant Protein

Isotype: IgG

Application Details

Application Notes:

Application Note: This affinity purified antibody has been tested for use in IF, and western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band 39-42 kDa for non reduced and 20-27 kDa for reduced gels corresponding to NGP by western blotting in the appropriate cell lysate or extract. 

Western Blot Dilution: 1:1000

IF Microscopy Dilution: 1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.1 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening.  Aliquot contents and freeze at -20° C or below for extended storage.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-Ngp antibody (ABIN5706813)

Hong, Qu, Wuest, Huang, Huang, Lin: "Neutrophilic Granule Protein Is a Novel Murine LPS Antagonist." in: Immune network, Vol. 19, Issue 5, pp. e34, (2019) (PubMed).

Target Details for Ngp

Target: Ngp (NGP) (Neutrophilic Granule Protein (NGP))

Alternative Name: Ngp

Background: Background: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI). A proteomic study in 2011 determined a direct correlation between NGP mRNA levels and myeloid-derived suppressor cells. Specifically cathepsin B, a catalytic mechanism and potentially biomarker in a variety of cancers and amyloid plaque development, was inhibited by NGP. The significance of NGP as a negative regulator of tumor metastasis makes this antibody ideal for investigators involved in cancer research, as well as Alzheimer's research.

Gene ID: 18054

NCBI Accession: NP_032720