NF-kB p65 antibody (acLys310)

Catalog No. ABIN5706804

Supplier Product No.: 600-401-j88

Product Details anti-NF-kB p65 Antibody

Target: NF-kB p65 (NFkBP65) (Nuclear Factor-kB p65 (NFkBP65))

Binding Specificity: acLys310

Reactivity: Human, Mouse, Rat, Chicken, Primate

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NF-kB p65 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Supplier Product No.: 600-401-j88

Supplier: Rockland

Purpose: NFkB [ac Lys 310] Antibody

Cross-Reactivity (Details): A BLAST analysis was used to suggest cross-reactivity with mouse and human based on 100 % sequence homology.

Characteristics: Synonyms: rabbit anti-NFKB Ac Lys310 antibody, rabbit anti-NfKB K310ac antibody, NFKB, NFkß, NF-kB, NF-kappaB, NFkappaB, Transcription factor p65, Nuclear factor NF-kappa-B p65 subunit, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3, RELA, NFKB3

Purification: Anti-NFkB [ac Lys 310] Antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Sterility: Sterile filtered

Immunogen:

Immunogen: Anti-NFkB K310ac affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic acetylated peptide surrounding Lysine 310 of human TF65 protein.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: User Optimized

Application Note: Anti-NFkB [ac Lys 310] Antibody has been tested in ELISA. Anti-NFkB K310ac is useful for in Immunohistochemistry and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~65 kDa corresponding to the appropriate cell lysate or extract.

Western Blot Dilution: 1 μg/mL

ELISA Dilution: 1:50,000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.05 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol with 1 mg/mL Bovine Serum Albumin (BSA)

Preservative: None

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for NF-kB p65

Target: NF-kB p65 (NFkBP65) (Nuclear Factor-kB p65 (NFkBP65))

Alternative Name: RELA (NFkBP65 Products)

Background: Background: NFkB is a pleiotropic transcription factor present in most cell types and is the endpoint of a series of signal transduction events related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorgenesis and apoptosis. NFkB is contained in the cytoplasm inactively until it is released by a phosphorylated I-kappa-B, allowing the NFkB to translocate to the nucleus. This antibody is ideal for investigators interested in Metabolism, Cancer, Cardiovascular, Microbiology, Epigenetics and Nuclear Signaling, Immunology, Neuroscience, or Cell Biology research.

UniProt: Q04206

Pathways: NF-kappaB Signaling, RTK Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades, S100 Proteins