Histone 3 antibody (H3K9me3, pThr6)

Catalog No. ABIN5706788

Supplier Product No.: 600-401-i66

Product Details anti-Histone 3 Antibody

Target: Histone 3 (H3)

Binding Specificity: H3K9me3, pThr6

Reactivity: Human, C. elegans

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Histone 3 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Chromatin Immunoprecipitation (ChIP), Dot Blot (DB), Fluorescence Microscopy (FM)

Supplier Product No.: 600-401-i66

Supplier: Rockland

Purpose: Histone H3 K9me3/phospho T6 Antibody

Cross-Reactivity (Details): This antibody reacts with human Histone H3.

Characteristics: Synonyms: rabbit anti-Histone H3 trimethyl Lys9 pT6 antibody, H3.3B, H3 histone, family 3A, H3.3AH3F3H3F3B, histone H3.3, MGC87783, MGC87782, H3K9me3/pT6

Purification: Anti-Histone H3 [Trimethyl Lys9, p Thr6] was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Sterility: Sterile filtered

Immunogen:

Immunogen: Histone H3 [Trimethyl Lys9, p Thr6] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic trimethylated/phosphorylated peptide surrounding Lysine 9/Threonine 6 of human Histone H3.2.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:200

Application Note: Anti-Histone H3 [Trimethyl Lys9, p Thr6] antibody is tested for Western Blot, Immunocytochemistry, Immunofluorescence, Chromatin Immunoprecipitation, and Dot Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~15.4 kDa corresponding to Histone H3 protein by Western Blotting in HeLa histone prep lysate or the appropriate cell lysate or extract. Epi-Plus™ antibody production in collaboration with Novus Biologicals.

ChIP Dilution: 2-5 μg/million cells

Western Blot Dilution: 1:500

IF Microscopy Dilution: 1:200

Other: Dot Blot 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 30 % Glycerol

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for Histone 3

Target: Histone 3 (H3)

Alternative Name: Histone H3 (H3 Products)

Background: Background: H3K9 methylations are a conserved hallmark of heterochromatin binding domains. The trimethyl K9 version of histone H3, when coupled with a T6 phosphorylation, is related to the Oct4 and Nanog proteins in embryoid bodies. This association seems to indicate a role for this modified histone in cellular differentiation in development. The formation of this modification seems to be a result of a complicated multiple silencing process for downstream genes, which is RNAi independent. Anti-Histone H3 are ideal for researchers interested in Chromatin Modifiers, Chromatin Research, Histones and Modified Histones, and Epigenetics research.

Gene ID: 126961

NCBI Accession: NP_001005464

UniProt: Q71DI3