POLQ antibody (Internal Region)
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- Target See all POLQ Antibodies
- POLQ (Polymerase (DNA Directed), theta (POLQ))
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Binding Specificity
- Internal Region
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Reactivity
- Human
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- This POLQ antibody is un-conjugated
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Application
- ELISA
- Purpose
- DNA polymerase theta
- Sequence
- DTHQQLTCSN DES
- Cross-Reactivity
- Human
- Purification
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Grade
- Recent
- Immunogen
- Peptide with sequence DTHQQLTCSNDES, from the internal region of the protein sequence according to NP_955452.3.
- Isotype
- IgG
- Top Product
- Discover our top product POLQ Primary Antibody
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- Application Notes
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Western Blot: Preliminary experiments in Human Brain (Cerebellum lysates) and in lysates of cell line HeLa gave no specific signal but low background (at antibody concentration up to 1 μg/mL).
Peptide ELISA: antibody detection limit dilution 1:1000.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Minimize freezing and thawing.
- Storage
- -20 °C
- Storage Comment
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- Target
- POLQ (Polymerase (DNA Directed), theta (POLQ))
- Alternative Name
- POLQ (POLQ Products)
- Synonyms
- POLH antibody, PRO0327 antibody, A430110D14Rik antibody, DNA polymerase theta antibody, polymerase (DNA directed), theta antibody, POLQ antibody, polq antibody, LOC100179525 antibody, Polq antibody
- Background
- POLQ , polymerase (DNA directed), theta , DKFZp781A0112, POLH, PRO0327, DNA polymerase eta, DNA polymerase theta
- Gene ID
- 10721
- NCBI Accession
- NP_955452
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