Histone 3 antibody (H3K4me3, H3K9ac)

Catalog No. ABIN5706769

Supplier Product No.: 600-401-j98

Product Details anti-Histone 3 Antibody

Target: Histone 3 (H3)

Binding Specificity: H3K4me3, H3K9ac

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Histone 3 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Dot Blot (DB), Multiplex Assay (MA), Fluorescence Microscopy (FM)

Supplier Product No.: 600-401-j98

Supplier: Rockland

Purpose: Histone H3 K4me3/K9ac Antibody

Cross-Reactivity (Details): A BLAST analysis was used to suggest cross-reactivity with Human, mouse, rat, and C.

Characteristics: Synonyms: rabbit anti-Histone H3 trimethyl Lys4 Ac Lys9 antibody, H3.3B, H3 histone, family 3A, H3.3AH3F3H3F3B, histone H3.3, MGC87783, MGC87782, H3 K4me3K9ac

Purification: Anti-Histone H3 [Trimethyl Lys4/ac Lys9] was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Sterility: Sterile filtered

Immunogen:

Immunogen: Anti-Histone H3 K4-Me3/K9-Ac affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide with a methylation surrounding Lysine 4 and an acetylation surrounding Lysine 9 of human Histone H3.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Application Note: Anti-Histone H3 K4-Me3/K9-Ac antibody is tested for dot blot, IF, and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~15.4 kDa corresponding to the appropriate cell lysate or extract. Epi-Plus™ antibody production in collaboration with Novus Biologicals.

Western Blot Dilution: 1:1000

IF Microscopy Dilution: 1:10

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.46 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 30 % Glycerol

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for Histone 3

Target: Histone 3 (H3)

Alternative Name: Histone H3 (H3 Products)

Background: Background: Histones of the nucleosome build chromatin and undergo various post-translational modifications proven to regulate chromatin condensation and DNA accessibility. Histone H3 methylations on Lysine 4 is modified by Set7/9 enzyme and is associated with transcriptional activation. Acetylated Lysine 9 has been studied in regards to transcriptional activation and histone deposition. Anti-Histone H3 K4-Me3/K9-Ac antibody is ideal for researchers interested in Chromatin Modifiers, Chromatin Research, Histones and Modified Histones, and Epigenetics research.

UniProt: Q71DI3