HBG1 antibody (N-Term)

Catalog No. ABIN5706737

Supplier Product No.: 200-301-gs6

Product Details anti-HBG1 Antibody

Target: HBG1 (Hemoglobin, gamma A (HBG1))

Binding Specificity: N-Term

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This HBG1 antibody is un-conjugated

Application: ELISA, Western Blotting (WB)

Supplier Product No.: 200-301-gs6

Supplier: Rockland

Purpose: Hemoglobin beta F Antibody

Cross-Reactivity (Details): This protein A purified mouse monoclonal antibody reacts specifically with human HbF gamma isoform.

Characteristics: Synonyms: mouse anti-HbF antibody, mouse anti-hemoglobin antibody, Gamma-1-globin, Hb F Agamma, Hemoglobin gamma-1 chain, Hemoglobin gamma-A chain, HBG1, HBG2, HbF Antibody, Sickle Cell Disease (SCD)

Purification: Anti-HbF is purified from tissue culture supernatant by protein A purification.

Sterility: Sterile filtered

Immunogen:

Immunogen: Anti-Hemoglobin beta F Monoclonal Antibody was produced in mice by repeated immunizations with synthetic peptide corresponding to amino acid residues near the N-terminus of Hb beta-subunit conjugated to KLH.

Immunogen Type: Conjugated Peptide

Clone: 4B3-B5-F3-B7

Isotype: IgG1 kappa

Application Details

Application Notes:

Application Note: Anti-Hemoglobin beta F (MOUSE) antibody has been tested ELISA and Western Blotting. This antibody is designed for use in lateral flow. Specific conditions of reactivity should be optimized by the end user. Expect a band of approximately 16 kDa.

Western Blot Dilution: 1 μg/mL

ELISA Dilution: 1:20,000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. This product is stable at 4° C as an undiluted liquid. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for HBG1

Target: HBG1 (Hemoglobin, gamma A (HBG1))

Alternative Name: HBG1 (HBG1 Products)

Background: Background: HbF antibodies detect the hemoglobin gamma isoform subunit. Functional alternate hemoglobin (Hb) is a hetero tetramer composed of 2 alpha and 2 gamma subunits (alpha-2 gamma-2). Hemoglobin F is elevated in newborns, reaching adult levels by 12 months. HbF levels are increased to as much as 5 % to 10 % in normal pregnancy. Sickle cell disease (SCD), thalassemias and hemoglobinopathies occur when aberrant forms of hemoglobin are expressed in children and adults. Hemoglobin variants arise from mutations in the globin genes and sickle cell disease and the more benign sickle cell trait are observed in more than 100 million people globally. HbF antibody does not react other forms of Hb. This antibody is ideal for investigators involved in Cardiovascular and developmental biology research.

Gene ID: 3047

NCBI Accession: NP_000550

UniProt: P69891