HBd antibody (N-Term)

Catalog No. ABIN5706735

Supplier Product No.: 200-301-gs8

Product Details anti-HBd Antibody

Target: HBd (Hemoglobin, delta (HBd))

Binding Specificity: N-Term

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This HBd antibody is un-conjugated

Application: ELISA, Western Blotting (WB)

Supplier Product No.: 200-301-gs8

Supplier: Rockland

Purpose: Hemoglobin beta A-2 Antibody

Cross-Reactivity (Details): This protein A purified mouse monoclonal antibody reacts specifically with human HbA-2 delta isoform.

Characteristics: Synonyms: mouse anti-HbA-2 antibody, mouse anti-hemoglobin antibody, Hemoglobin Subunit Delta, Hemoglobin Delta Chain 4, Delta-Globin 4, HbD, HbA-2 Antibody, Sickle Cell Disease (SCD)

Purification: Anti-HbA-2 is purified from tissue culture supernatant by protein A purification.

Sterility: Sterile filtered

Immunogen:

Immunogen: Anti-Hemoglobin beta A-2 Monoclonal Antibody was produced in mice by repeated immunizations with synthetic peptide corresponding to amino acid residues near the N-terminus of Hb delta-subunit conjugated to KLH.

Immunogen Type: Conjugated Peptide

Clone: 21G1-F1-B9-G9-D11

Isotype: IgG1 kappa

Application Details

Application Notes:

Application Note: Anti-Hemoglobin beta A-2 (MOUSE) antibody has been tested by ELISA and Western Blotting. This antibody is designed for use in lateral flow. Specific conditions of reactivity should be optimized by the end user. Expect a band of approximately 16 kDa.

Western Blot Dilution: 1 μg/mL

ELISA Dilution: 1:20,000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.00 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. This product is stable at 4° C as an undiluted liquid. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for HBd

Target: HBd (Hemoglobin, delta (HBd))

Alternative Name: HBD (HBd Products)

Background: Background: HbA-2 or hemoglobin delta subunit antibodies detect the delta-specific sequence in the hemoglobin delta-subunit found in HbA-2. Functional hemoglobin (Hb) is a hetero tetramer and the dominant form of Adult Hb is composed of 2 alpha and 2 beta subunits (α2β2). Hemoglobin A-2 (HbA-2) is a normal but minor variant of hemoglobin A that consists of two alpha and two delta chains (α2δ2). Hemoglobin A-2 may be increased in beta thalassemia or in people who are heterozygous for the beta thalassemia gene, and HbA2 is also linked to neurological disorders. HbA-2 form exists in small amounts in all adult humans (1.5-3.1 % of all hemoglobin molecules) and is increased in people with Sickle-cell disease. Its normal biological role is not well understood. HbA-2 antibody does not react other forms of Hb including no cross-reaction to HbA or beta subunit. This antibody is ideal for investigators involved in Cardiovascular and developmental biology research.

Gene ID: 3045

NCBI Accession: NP_000510

UniProt: P02042