NF-kB p65 antibody (AA 99-551)

Catalog No. ABIN5693244

This Rabbit Polyclonal antibody specifically detects NF-kB p65 in WB, ELISA, IF, FACS and ICC. It exhibits reactivity toward Human, Mouse and Rat and has been mentioned in 45+ publications.

Quick Overview for NF-kB p65 antibody (AA 99-551) (ABIN5693244)

Target: NF-kB p65 (NFkBP65) (Nuclear Factor-kB p65 (NFkBP65))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NF-kB p65 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Product Details anti-NF-kB p65 Antibody

Binding Specificity: AA 99-551

Purpose: Anti-NF-kB p65/RELA Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-NF-kB p65/RELA Antibody Picoband® (ABIN5693244). Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Immunogen: E. coli-derived human NF-kB p65 recombinant protein (Position: F99-S551).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL
Immunocytochemistry/Immunofluorescence, 5 μg/mL
Immunoprecipitation, 0.5-2 μg/mL
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells
ELISA, 0.1-0.5 μg/mL
1. Nolan GP, Ghosh S, Liou HC, Tempst P, Baltimore D (April 1991). "DNA binding and I kappa B inhibition of the cloned p65 subunit of NF-kappa B, a rel-related polypeptide". Cell 64 (5): 961-9. 2. Lim, C.-A., Yao, F., Wong, J. J.-Y., George, J., Xu, H., Chiu, K. P., Sung, W.-K., Lipovich, L., Vega, V. B., Chen, J., Shahab, A., Zhao, X. D., Hibberd, M., Wei, C.-L., Lim, B., Ng, H.-H., Ruan, Y., Chin, K.-C. Genome-wide mapping of RELA(p65) binding identifies E2F1 as a transcriptional activator recruited by NF-kappa-B upon TLR4 activation.Molec. Cell 27: 622-635, 2007. 3. Mazzeo, A., Aguennouz, M., Messina, C., Vita, G. Immunolocalization and activation of transcription factor nuclear factor kappa-B in dysimmune neuropathies and familial amyloidotic polyneuropathy. Arch. Neurol. 61: 1097-1102, 2004.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

References for anti-NF-kB p65 antibody (ABIN5693244)

"Retraction: Naringin Alleviates Diabetic Kidney Disease through Inhibiting Oxidative Stress and Inflammatory Reaction." in: PLoS ONE, Vol. 13, Issue 2, pp. e0192465, (2018) (PubMed).

Wang, Lv, Guo, Duan, Dong, Wang, Yu, Su, Kuang: "Pharmacological Effect of Caulophyllum robustum on Collagen-Induced Arthritis and Regulation of Nitric Oxide, NF-κB, and Proinflammatory Cytokines In Vivo and In Vitro." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2017, pp. 8134321, (2018) (PubMed).

Liu, Zhang, Han, Wang, Liu, Zhang, Zhou, Xiang: "[Corrigendum] Inhibition of BTK protects lungs from trauma-hemorrhagic shock-induced injury in rats." in: Molecular medicine reports, Vol. 17, Issue 5, pp. 6926, (2018) (PubMed).

Wang, Liu, Wang, Wang, Tang, Jiang: "Src Promotes Metastasis of Human Non-Small Cell Lung Cancer Cells through Fn14-Mediated NF-κB Signaling." in: Medical science monitor : international medical journal of experimental and clinical research, Vol. 24, pp. 1282-1294, (2018) (PubMed).

Liu, Bao, Zeng, Wei: "Effects of resveratrol and genistein on nuclear factor‑κB, tumor necrosis factor‑α and matrix metalloproteinase‑9 in patients with chronic obstructive pulmonary disease." in: Molecular medicine reports, Vol. 13, Issue 5, pp. 4266-72, (2017) (PubMed).

Yu, Wang, He, Wang, Gao, Ren, Shi et al.: "Curcumin exerts anti-inflammatory and antioxidative properties in 1-methyl-4-phenylpyridinium ion (MPP(+))-stimulated mesencephalic astrocytes by interference with TLR4 and downstream signaling ..." in: Cell stress & chaperones, Vol. 21, Issue 4, pp. 697-705, (2017) (PubMed).

Wu, Gao, Li, Yao, Wang, Chen, Li, Ma, Ji, Li: "Hydrogen Sulfide Mitigates Kidney Injury in High Fat Diet-Induced Obese Mice." in: Oxidative medicine and cellular longevity, Vol. 2016, pp. 2715718, (2017) (PubMed).

Xie, Zhong, Li: "Comment on "Puerarin Improves Diabetic Aorta Injury by Inhibiting NADPH Oxidase-Derived Oxidative Stress in STZ-Induced Diabetic Rats"." in: Journal of diabetes research, Vol. 2016, pp. 7302620, (2017) (PubMed).

Sun, Yang, Zhang, Zhao: "Esculentoside A ameliorates cecal ligation and puncture-induced acute kidney injury in rats." in: Experimental animals, (2017) (PubMed).

Yang, Gao, Wu, Yu, Li, Meng, Li, Yan, Jin: "Epigallocatechin-3-gallate attenuates neointimal hyperplasia in a rat model of carotid artery injury by inhibition of high mobility group box 1 expression." in: Experimental and therapeutic medicine, Vol. 14, Issue 3, pp. 1975-1982, (2017) (PubMed).

Zhao, Ding, Liu, Yin, Zhang, Ma: "Unfractionated heparin protects the protein C system against lipopolysaccharide-induced damage in vivo and in vitro." in: Experimental and therapeutic medicine, Vol. 14, Issue 6, pp. 5515-5522, (2017) (PubMed).

Chen, Liu, Wang, Feng, Zhao, Shi, Liu: "Protective Effects of Hydrogen-Rich Saline on Rats with Smoke Inhalation Injury." in: Oxidative medicine and cellular longevity, Vol. 2015, pp. 106836, (2016) (PubMed).

Huang, Zhong, Zhang, Wang, Xu, Zhu, Wang, Hu, Zhao: "Down-regulation of HMGB1 expression by shRNA constructs inhibits the bioactivity of urothelial carcinoma cell lines via the NF-κB pathway." in: Scientific reports, Vol. 5, pp. 12807, (2016) (PubMed).

Tang, Gao, Mao, He, Liu, Cai, Lin, Wu: "Salidroside protects against bleomycin-induced pulmonary fibrosis: activation of Nrf2-antioxidant signaling, and inhibition of NF-κB and TGF-β1/Smad-2/-3 pathways." in: Cell stress & chaperones, Vol. 21, Issue 2, pp. 239-49, (2016) (PubMed).

Zuo, Zheng, Liu, Ma: "Fenofibrate, a PPARα agonist, protect proximal tubular cells from albumin-bound fatty acids induced apoptosis via the activation of NF-kB." in: International journal of clinical and experimental pathology, Vol. 8, Issue 9, pp. 10653-61, (2016) (PubMed).

Wendan, Changzhu, Xuhong, Hongjing, Hong, Dongxia, Fang: "BMSCs Interactions with Adventitial Fibroblasts Display Smooth Muscle Cell Lineage Potential in Differentiation and Migration That Contributes to Neointimal Formation." in: Stem cells international, Vol. 2016, pp. 3196071, (2016) (PubMed).

Li, Zhao, Wu, Lu, Shi, Chen: "Puerarin Improves Diabetic Aorta Injury by Inhibiting NADPH Oxidase-Derived Oxidative Stress in STZ-Induced Diabetic Rats." in: Journal of diabetes research, Vol. 2016, pp. 8541520, (2016) (PubMed).

Wang, Li, Zhen, Xu, Sun: "Simvastatin ameliorates renal lipidosis through the suppression of renal CXCL16 expression in mice with adriamycin-induced nephropathy." in: International journal of clinical and experimental pathology, Vol. 8, Issue 12, pp. 15696-707, (2016) (PubMed).

Zhao, Zhao, Wang, Li, Zhang: "Glycyrrhizic Acid Attenuates Sepsis-Induced Acute Kidney Injury by Inhibiting NF-κB Signaling Pathway." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2016, pp. 8219287, (2016) (PubMed).

Ge, Cao, Wang, Sun, Sun, Han, Cui, Liu: "Overexpression of Livin promotes migration and invasion of colorectal cancer cells by induction of epithelial-mesenchymal transition via NF-κB activation." in: OncoTargets and therapy, Vol. 9, pp. 1011-21, (2016) (PubMed).

Target Details for NF-kB p65

Target: NF-kB p65 (NFkBP65) (Nuclear Factor-kB p65 (NFkBP65))

Alternative Name: RELA

Background:

Synonyms: Transcription factor p65, Nuclear factor NF-kappa-B p65 subunit, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3, RELA, NFKB3

Tissue Specificity: Widely expressed. Isoform 1 and isoform 3 are predominant. Isoform 4, isoform 5 and isoform 6 are expressed moderately. Isoform 8 is found at low frequency. Isoform 7, isoform 9 and isoform 10 are not expressed in adult tissues, with the exception of adult retina expressing isoform 10. Isoform 9 is faintly expressed in fetal brain, heart, lung, skeletal muscle and spleen. Fetal thymus expresses isoforms 1, 7, 9 and 10 at similar levels.

Background: Transcription factor p65, also known as NFKB3 or NF-kB p65, is a protein that in humans is encoded by the RELA gene. It is mapped to 11q13.1. NFKB is an essential transcription factor complex involved in all types of cellular processes, including cellular metabolism, chemotaxis, etc, and it may play a role in inflammatory conditions of the peripheral nervous system. Phosphorylation and acetylation of NFKB3 are crucial post-translational modifications required for NFKB activation. It has also been shown to modulate immune responses, and activation of NFKB3 is positively associated with multiple types of cancer. In addition to that, NFKB3 antagonizes TNFR1-JNK proliferative signals in epidermis and plays a nonredundant role in restraining epidermal growth.

Molecular Weight: 65 kDa

Gene ID: 5970

UniProt: Q04206

Pathways: NF-kappaB Signaling, RTK Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades, S100 Proteins