NSF antibody (AA 620-744)

Catalog No. ABIN5693126

Product Details anti-NSF Antibody

Target: NSF (N-Ethylmaleimide-Sensitive Factor (NSF))

Binding Specificity: AA 620-744

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NSF antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Brand: Picoband™

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for NSF detection. Tested with WB, IHC-P, Direct ELISA in Human,Mouse,Rat.

Immunogen: E. coli-derived human NSF recombinant protein (Position: N620-D744).

Application Details

Application Notes:

Recommended Detection Systems: Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ABIN921124) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).

Application Details: Western blot, 0.1-0.5 μg/mL
Immunohistochemistry(Paraffin-embedded Section), 0.5-1 μg/mL
Direct ELISA, 0.1-0.5 μg/mL

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Target Details for NSF

Target: NSF (N-Ethylmaleimide-Sensitive Factor (NSF))

Alternative Name: NSF (NSF Products)

Synonyms: SKD2 antibody, AI316878 antibody, AU020090 antibody, AU067812 antibody, NSF antibody, 18.m06598 antibody, nsf antibody, si:bz18k17.1 antibody, wu:fj33g11 antibody, N-ethylmaleimide sensitive factor antibody, T1J1.4 antibody, T1J1_4 antibody, NEM-sensitive fusion protein antibody, N-ethylmaleimide sensitive factor, vesicle fusing ATPase antibody, N-ethylmaleimide sensitive fusion protein antibody, N-ethylmaleimide-sensitive factor antibody, N-ethylmaleimide sensitive factor antibody, N-ethylmaleimide-sensitive fusion protein, putative antibody, N-ethylmaleimide sensitive factor L homeolog antibody, vesicle-fusing ATPase antibody, N-ethylmaleimide-sensitive factor a antibody, AAA-type ATPase family protein antibody, NSF antibody, Nsf antibody, nsf antibody, BBOV_II007200 antibody, TGME49_318510 antibody, nsf.L antibody, LOC100180685 antibody, LOC9310886 antibody, nsfa antibody

Background:

Synonyms: Vesicle-fusing ATPase, N-ethylmaleimide-sensitive fusion protein, NEM-sensitive fusion protein, Vesicular-fusion protein NSF, NSF

Background: N-ethylmaleimide-sensitive factor, also known as NSF, is an enzyme which in humans is encoded by the NSF gene. NSF is a homohexameric AAA ATPase involved in membrane fusion. NSF is ubiquitously found in the cytoplasm of eukaryotic cells. It is a central component of the cellular machinery in the transfer of membrane vesicles from one membrane compartment to another. During this process, SNARE proteins on two joining membranes (usually a vesicle and a target membrane such as the plasma membrane) form a tight complex. This aids fusion of the vesicle with the target membrane. It has been proposed that the role of NSF is to undo these SNARE complexes once membrane fusion has occurred, using the hydrolysis of ATP as an energy source, allowing the dissociated SNAREs to be recycled for reuse in further rounds of membrane fusion. This proposal remains controversial, however. Recent work indicates that the ATPase function of NSF does not function in recycling of vesicles but rather functions in the act of fusing vesicles with the plasma membrane.

UniProt: P46459