MMP13 antibody (AA 99-335)

Catalog No. ABIN5693125

Product Details anti-MMP13 Antibody

Target: MMP13 (Matrix Metallopeptidase 13 (Collagenase 3) (MMP13))

Binding Specificity: AA 99-335

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MMP13 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Brand: Picoband™

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for MMP13 detection. Tested with WB, IHC-P, Direct ELISA in Human,Mouse,Rat.

Immunogen: E. coli-derived rat MMP13 recombinant protein (Position: Y99-H335).

Application Details

Application Notes:

Recommended Detection Systems: Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ABIN921124) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).

Application Details: Western blot, 0.1-0.5 μg/mL
Immunohistochemistry(Paraffin-embedded Section), 0.5-1 μg/mL
Direct ELISA, 0.1-0.5 μg/mL

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

References for anti-MMP13 antibody (ABIN5693125)

Ma, Lv, Yu, Zhang, Kong, Niu, Yi: "Protective effects of tumor necrosis factor-α blockade by adalimumab on articular cartilage and subchondral bone in a rat model of osteoarthritis." in: Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas, Vol. 48, Issue 10, pp. 863-70, (2016) (PubMed).

Liu, Tian, Zhou, Wang, Gou, Zhang, Wang, Shen, Zhang, Zhang: "Protective effect of calcitonin on lumbar fusion-induced adjacent-segment disc degeneration in ovariectomized rat." in: BMC musculoskeletal disorders, Vol. 16, pp. 342, (2016) (PubMed).

Xia, He, Guo, Qing, He: "Effects of ultrasound on estradiol level, bone mineral density, bone biomechanics and matrix metalloproteinase-13 expression in ovariectomized rabbits." in: Experimental and therapeutic medicine, Vol. 10, Issue 4, pp. 1429-1436, (2015) (PubMed).

Huang, Zou, Shi, Zhang, Pen, Zhang, Gao, Wang: "The effect of electroacupuncture on the extracellular matrix synthesis and degradation in a rabbit model of disc degeneration." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2014, pp. 731395, (2014) (PubMed).

Target Details for MMP13

Target: MMP13 (Matrix Metallopeptidase 13 (Collagenase 3) (MMP13))

Alternative Name: Mmp13 (MMP13 Products)

Synonyms: CLG3 antibody, MANDP1 antibody, Clg antibody, MMP-13 antibody, Mmp1 antibody, cb1034 antibody, mmp13 antibody, gene A antibody, xCol antibody, xcl3 antibody, MMP13 antibody, MGC108008 antibody, matrix metallopeptidase 13 antibody, matrix metallopeptidase 13a antibody, matrix metallopeptidase 13 (collagenase 3) like S homeolog antibody, matrix metallopeptidase 13 (collagenase 3) antibody, matrix metalloproteinase 13 antibody, matrix metallopeptidase 13 (collagenase 3) like L homeolog antibody, MMP13 antibody, Mmp13 antibody, mmp13a antibody, mmp13l.S antibody, mmp13 antibody, LOC100136348 antibody, mmp13l.L antibody

Background:

Synonyms: Collagenase 3, Matrix metalloproteinase-13, MMP-13, UMRCASE, Mmp13

Background: Collagenase 3 is an enzyme that in humans is encoded by the MMP13 gene. This gene encodes a member of the peptidase M10 family of matrix metalloproteinases (MMPs). Proteins in this family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. The encoded preproprotein is proteolytically processed to generate the mature protease. This protease cleaves type II collagen more efficiently than types I and III. It may be involved in articular cartilage turnover and cartilage pathophysiology associated with osteoarthritis. Mutations in this gene are associated with metaphyseal anadysplasia. This gene is part of a cluster of MMP genes on chromosome 11.

UniProt: P23097