PPID antibody (AA 306-370)

Catalog No. ABIN5693052

Product Details anti-PPID Antibody

Target: PPID (Peptidylprolyl Isomerase D (PPID))

Binding Specificity: AA 306-370

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PPID antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunocytochemistry (ICC), Flow Cytometry (FACS)

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for PPID detection. Tested with WB, IHC-P, IHC-F, ICC, FCM, Direct ELISA in Human,Mouse,Rat.

Immunogen: E. coli-derived human PPID recombinant protein (Position: N306-A370).

Application Details

Application Notes:

Recommended Detection Systems: Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ABIN921124) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P), IHC(F) and ICC.

Application Details: Western blot, 0.1-0.5 μg/mL
Immunohistochemistry(Paraffin-embedded Section), 0.5-1 μg/mL
Immunohistochemistry(Frozen Section), 0.5-1 μg/mL
Immunocytochemistry, 0.5-1 μg/mL
Flow Cytometry, 1-3 μg/1x10⁶ cells
Direct ELISA, 0.1-0.5 μg/mL

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Target Details for PPID

Target: PPID (Peptidylprolyl Isomerase D (PPID))

Alternative Name: PPID (PPID Products)

Synonyms: PPID antibody, CYP-40 antibody, CYPD antibody, Cyp-40 antibody, CypD antibody, wu:fb18b07 antibody, zgc:86711 antibody, 4930564J03Rik antibody, Ppidl antibody, Ppif antibody, cyp-40 antibody, cypd antibody, peptidylprolyl isomerase D antibody, peptidylprolyl isomerase D (cyclophilin D) antibody, peptidylprolyl isomerase D (cyclophilin D) S homeolog antibody, PPID antibody, Ppid antibody, ppid antibody, ppid.S antibody

Background:

Synonyms: Peptidyl-prolyl cis-trans isomerase D, PPIase D

Tissue Specificity: Widely expressed.

Background: Cyclophilin D, Peptidylprolyl isomerase D, also known as PPID, is an enzyme which in humans is encoded by the PPID gene. The protein encoded by this gene is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. The Cyclophilin D (PPID) gene contains 10 exons and spans 14.2 kb of genomic DNA. By fluorescence in situ hybridization, the PPID gene is mapped to chromosome 4q31.3. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. This protein has been shown to possess PPIase activity and, similar to other family members, can bind to the immunosuppressant ciclosporin.

UniProt: Q08752

Pathways: Nuclear Hormone Receptor Binding