PARN antibody (AA 1-301)

Catalog No. ABIN5693032

Product Details anti-PARN Antibody

Target: PARN (Poly A Specific Ribonuclease (PARN))

Binding Specificity: AA 1-301

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PARN antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Brand: Picoband™

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for PARN detection. Tested with WB, IHC-P, Direct ELISA in Human,Mouse,Rat.

Immunogen: E. coli-derived human PARN recombinant protein (Position: M1-Y301).

Application Details

Application Notes:

Recommended Detection Systems: Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ABIN921124) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).

Application Details: Western blot, 0.1-0.5 μg/mL
Immunohistochemistry(Paraffin-embedded Section), 0.5-1 μg/mL
Direct ELISA, 0.1-0.5 μg/mL

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Target Details for PARN

Target: PARN (Poly A Specific Ribonuclease (PARN))

Alternative Name: PARN (PARN Products)

Synonyms: parn-A antibody, xparn antibody, DAN antibody, RGD1565449 antibody, zgc:56067 antibody, 1200003I18Rik antibody, poly(A)-specific ribonuclease L homeolog antibody, poly(A)-specific ribonuclease antibody, poly(A)-specific ribonuclease (deadenylation nuclease) antibody, parn.L antibody, PARN antibody, Parn antibody, parn antibody

Background:

Synonyms: Poly(A)-specific ribonuclease PARN, Deadenylating nuclease, Deadenylation nuclease, Polyadenylate-specific ribonuclease, PARN, DAN

Tissue Specificity: Ubiquitous.

Background: Poly(A)-specific ribonuclease (PARN), also known as polyadenylate-specific ribonuclease or deadenylating nuclease (DAN), is an enzyme that in humans is encoded by the PARN gene. The protein encoded by this gene is a 3'-exoribonuclease, with similarity to the RNase D family of 3'-exonucleases. It prefers poly(A) as the substrate, hence, efficiently degrades poly(A) tails of mRNAs. Exonucleolytic degradation of the poly(A) tail is often the first step in the decay of eukaryotic mRNAs. This protein is also involved in silencing of certain maternal mRNAs during oocyte maturation and early embryonic development, as well as in nonsense-mediated decay (NMD) of mRNAs that contain premature stop codons.

UniProt: O95453