ICOS antibody (AA 21-200)

Catalog No. ABIN5693001

Product Details anti-ICOS Antibody

Target: ICOS (Inducible T-Cell Co-Stimulator (ICOS))

Binding Specificity: AA 21-200

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ICOS antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Brand: Picoband™

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for ICOS detection. Tested with WB, IHC-P, Direct ELISA in Human,Mouse,Rat.

Immunogen: E. coli-derived mouse ICOS recombinant protein (Position: E21-S200).

Application Details

Application Notes:

Recommended Detection Systems: Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ABIN921124) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).

Application Details: Western blot, 0.1-0.5 μg/mL
Immunohistochemistry(Paraffin-embedded Section), 0.5-1 μg/mL
Direct ELISA, 0.1-0.5 μg/mL

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Target Details for ICOS

Target: ICOS (Inducible T-Cell Co-Stimulator (ICOS))

Alternative Name: Icos (ICOS Products)

Synonyms: ICOS antibody, AILIM antibody, CCLP antibody, CRP-1 antibody, H4 antibody, Ly115 antibody, CD278 antibody, CVID1 antibody, Ailim antibody, inducible T-cell costimulator antibody, inducible T cell co-stimulator antibody, inducible T-cell co-stimulator antibody, ICOS antibody, LOC100232284 antibody, Icos antibody

Background:

Synonyms: Inducible T-cell costimulator, Activation-inducible lymphocyte immunomediatory molecule, CD28 and CTLA-4-like protein, CCLP, CD28-related protein 1, CRP-1, CD278, Icos, Ailim

Tissue Specificity: Expressed on activated T-cells and resting memory T-cells. High expression seen in the thymic medulla and in the germinal centers and T-cell zones of lymph nodes and Peyer patches. Expressed at low levels in the spleen.

Background: Inducible T-cell costimulator is an immune checkpoint protein that in humans is encoded by the ICOS gene. The protein encoded by this gene belongs to the CD28 and CTLA-4 cell-surface receptor family. It forms homodimers and plays an important role in cell-cell signaling, immune responses, and regulation of cell proliferation.

Pathways: Cancer Immune Checkpoints