CCKBR antibody

Catalog No. ABIN5692993

Product Details anti-CCKBR Antibody

Target: CCKBR (Cholecystokinin B Receptor (CCKBR))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CCKBR antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC)

Brand: Picoband™

Sequence: PVYTVVQPVG PRVLQCVHRW PSARVRQTWS

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for CCKBR detection. Tested with WB, IHC-F, ICC, FCM in Human,Mouse,Rat.

Immunogen: A synthetic peptide corresponding to a sequence of human CCKBR (PVYTVVQPVGPRVLQCVHRWPSARVRQTWS).

Application Details

Application Notes:

Recommended Detection Systems: Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ABIN921124) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(F) and ICC.

Application Details: Western blot, 0.1-0.5 μg/mL
Immunohistochemistry(Frozen Section), 0.5-1 μg/mL, Human
Immunocytochemistry, 0.5-1 μg/mL, Human
Flow Cytometry, 1-3 μg/1x10⁶ cells, Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

References for anti-CCKBR antibody (ABIN5692993)

Li, Huang, Liu: "Changes in FABP1 and gastrin receptor expression in the testes of rats that have undergone electrical injury." in: Experimental and therapeutic medicine, Vol. 9, Issue 6, pp. 2155-2158, (2015) (PubMed).

Target Details for CCKBR

Target: CCKBR (Cholecystokinin B Receptor (CCKBR))

Alternative Name: CCKBR (CCKBR Products)

Synonyms: CCKBR antibody, LOC100232835 antibody, CCK-B antibody, CCK2R antibody, GASR antibody, Cck2r antibody, Cholrec antibody, CCKR-2 antibody, CCK-BR antibody, CCK2-R antibody, CCK-CHR antibody, cholecystokinin B receptor antibody, cholecystokinin receptor-like antibody, CCKBR antibody, LOC100232835 antibody, Cckbr antibody

Background:

Synonyms: Gastrin/cholecystokinin type B receptor, CCK-B receptor, CCK-BR, Cholecystokinin-2 receptor, CCK2-R, CCKBR, CCKRB

Tissue Specificity: Isoform 1 is expressed in brain, pancreas, stomach, the colon cancer cell line LoVo and the T-lymphoblastoma Jurkat, but not in heart, placenta, liver, lung, skeletal muscle, kidney or the stomach cancer cell line AGS. Expressed at high levels in the small cell lung cancer cell line NCI-H510, at lower levels in NCI-H345, NCI-H69 and GLC-28 cell lines, not expressed in GLC-19 cell line. Within the stomach, expressed at high levels in the mucosa of the gastric fundus and at low levels in the antrum and duodenum. Isoform 2 is present in pancreatic cancer cells and colorectal cancer cells, but not in normal pancreas or colonic mucosa. Isoform 3 is expressed in brain, pancreas, stomach, the stomach cancer cell line AGS and the colon cancer cell line LoVo.

Background: The cholecystokinin B receptor, also known as CCKBR or CCK2, is a protein that in humans is encoded by the CCKBR gene. This gene encodes a G-protein coupled receptor for gastrin and cholecystokinin (CCK), regulatory peptides of the brain and gastrointestinal tract. This protein is a type B gastrin receptor, which has a high affinity for both sulfated and nonsulfated CCK analogs and is found principally in the central nervous system and the gastrointestinal tract. Alternative splicing results in multiple transcript variants. A misspliced transcript variant including an intron has been observed in cells from colorectal and pancreatic tumors.

UniProt: P32239

Pathways: Positive Regulation of Peptide Hormone Secretion, Feeding Behaviour